Glutamic-pyruvic transaminase electrochemical detection composition, application, electrochemical sensor and detection method

An alanine aminotransferase electrolytic and alanine aminotransferase technology, applied in the field of alanine aminotransferase electrochemical detection composition, can solve the problems of alanine aminotransferase detection interference, inaccurate detection results, etc.

Inactive Publication Date: 2019-08-16
GUANGZHOU WONDFO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there will be endogenous pyruvate in normal fingertip blood, and the presence of pyruvate

Method used

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  • Glutamic-pyruvic transaminase electrochemical detection composition, application, electrochemical sensor and detection method
  • Glutamic-pyruvic transaminase electrochemical detection composition, application, electrochemical sensor and detection method
  • Glutamic-pyruvic transaminase electrochemical detection composition, application, electrochemical sensor and detection method

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Experimental program
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Embodiment

[0049] Configure the first composition and the second composition according to Table 1 and Table 2:

[0050] Table 1 First Composition Components

[0051]

[0052]

[0053] Table 2 Second Composition Components

[0054] components content water 1000ml Tris-Hcl at pH 5-8 1.5g Sodium chloride 1g PEG8000 1g Cellulose 1g silica 1g Triton X-100 2.5g alpha-ketoglutarate 1g L-alanine 1g pyruvate oxidase 1g Potassium ferricyanide 2.5g

[0055] An insulating substrate 100 provided with electrodes is provided. The insulating substrate 100 is provided with a double branch flow channel 103 and two reaction pools, and a working electrode and a reference electrode are formed in the reaction area by screen printing. Spot the prepared first composition and second composition in different reaction areas respectively, and cover the working electrode. The insulating substrate 100 provided with the first co...

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Abstract

The present invention discloses a glutamic-pyruvic transaminase electrochemical detection composition. The composition comprises a first composition and a second composition. The first composition comprises water, a first buffer solution, a first electrolyte, a first surface active agent, a first electron mediator and pyruvate oxidase. The second composition comprises water, a second buffer solution, a second electrolyte, a second surface active agent, a second electron mediator, [alpha]- ketoglutaric acid, L- alanine and the pyruvate oxidase. The present invention also discloses an application of the glutamic-pyruvic transaminase electrochemical detection composition. The present invention also discloses a glutamic-pyruvic transaminase electrochemical sensor and a glutamic-pyruvic transaminase detection method.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to an electrochemical detection composition for alanine aminotransferase, its application, an electrochemical sensor and a detection method. Background technique [0002] Alanine aminotransferase is an important enzyme in human metabolism. Quantitative detection of the concentration of alanine aminotransferase in the human body can understand the current degree of liver function damage in the human body and judge the effect of treatment, and can also perform routine liver function testing on healthy people. [0003] Generally, the concentration of alanine aminotransferase in the human body is characterized by the detection of alanine aminotransferase in blood. Electrochemical sensors are one method used to detect the concentration of components in blood. The use of electrochemical sensors to detect the concentration of components can be tested by collecting fingertip blood. The...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/3277
Inventor 吴岸峰王继华
Owner GUANGZHOU WONDFO BIOTECH
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