Glutamic oxalacetic transaminase electrochemical detection composition and application thereof as well as electrochemical sensor and detection method

A technology for aspartate aminotransferase and aminotransferase electrolysis is applied in the field of electrochemical detection compositions for aspartate aminotransferase, and can solve the problems of inaccurate detection results and interference in detection of aspartate aminotransferase.

Inactive Publication Date: 2019-08-16
GUANGZHOU WONDFO BIOTECH
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  • Abstract
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  • Claims
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Problems solved by technology

However, there will be endogenous pyruvate in normal fingertip blood, and the presence of pyruvate w

Method used

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  • Glutamic oxalacetic transaminase electrochemical detection composition and application thereof as well as electrochemical sensor and detection method
  • Glutamic oxalacetic transaminase electrochemical detection composition and application thereof as well as electrochemical sensor and detection method
  • Glutamic oxalacetic transaminase electrochemical detection composition and application thereof as well as electrochemical sensor and detection method

Examples

Experimental program
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Embodiment

[0052] Configure the first composition and the second composition according to Table 1 and Table 2:

[0053] Table 1 First Composition Components

[0054] components content water 1000ml Tris-Hcl at pH 5-8 1.5g Sodium chloride 1g sodium alginate 1g PEG8000 1g Cellulose 1g silica 1g Triton X-100 2.5g pyruvate oxidase 1g Potassium ferricyanide 2.5g

[0055] Table 2 Second Composition Components

[0056]

[0057]

[0058] An insulating substrate 100 provided with electrodes is provided. The insulating substrate 100 is provided with a double branch flow channel 103 and two reaction pools, and a working electrode and a reference electrode are formed in the reaction area by screen printing. Spot the prepared first composition and second composition in different reaction areas respectively, and cover the working electrode. The insulating substrate 100 provided with the first composition and the secon...

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Abstract

The invention discloses a glutamic oxalacetic transaminase electrochemical detection composition, comprising a first composition and a second composition, wherein the first composition comprises water, first buffer solution, first electrolyte, a first surface active agent, a first electronic mediator and pyruvate oxidase, and the second composition comprises water, second buffer solution, second electrolyte, a second surface active agent, a second electronic mediator, L-aspartic acid, L-alanine, oxaloacetic acid decarboxylase and pyruvate oxidase. The invention also discloses application of the glutamic oxalacetic transaminase electrochemical detection composition. The invention also discloses a glutamic oxalacetic transaminase electrochemical sensor and a glutamic oxalacetic transaminasedetection method.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to an aspartate transaminase electrochemical detection composition, its application, an electrochemical sensor and a detection method. Background technique [0002] Aspartate aminotransferase is an important enzyme in human metabolism. Quantitative detection of aspartate aminotransferase concentration in the human body can understand the current degree of liver function damage in the human body and judge the effect of treatment, and can also perform routine liver function testing on healthy people. [0003] Generally, the concentration of human aspartate aminotransferase is characterized by the detection of aspartate aminotransferase in blood. Electrochemical sensors are one method used to detect the concentration of components in blood. The use of electrochemical sensors to detect the concentration of components can be tested by collecting fingertip blood. The blood volume req...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/3277
Inventor 吴岸峰王继华
Owner GUANGZHOU WONDFO BIOTECH
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