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Manganese ion diagnosis/ determination kit and method for determining manganese ion concentration

A technology of manganese ions and reagents, applied in the field of medical/food/environmental inspection and determination

Inactive Publication Date: 2010-01-06
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Neutron excitation analysis (NAA) is the best method, but can only be determined in some well-conditioned laboratories

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The manganese ion diagnosis / measurement reagent of the present embodiment is a single reagent, comprising:

[0040] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0041] Stabilizer 500mmol / L

[0042] Reduced coenzyme 0.25mmol / L

[0043] Oxaloacetate decarboxylase 6000U / L

[0044] Pyruvate dehydrogenase 8000U / L

[0045] Lactate dehydrogenase 10000U / L

[0046] Oxaloacetate 12mmol / L

[0047] Acetyl-CoA 6mmol / L

[0048] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0049] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test subwavelength 405nm, volume ratio of manganese ion sample to reagent is 1 / 25 , the reaction direction is negative reaction (decreasing reaction), the delay time is about 1 minute, and the detect...

Embodiment 2

[0052] The manganese ion diagnosis / measurement reagent of the present embodiment is a double reagent, comprising:

[0053] Reagent 1

[0054] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0055] Stabilizer 50mmol / L

[0056] Reduced coenzyme 0.25mmol / L

[0057] Oxaloacetate 12mmol / L

[0058] Acetyl-CoA 6mmol / L

[0059] Reagent 2

[0060] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0061] Stabilizer 500mmol / L

[0062] Oxaloacetate decarboxylase 6000U / L

[0063] Pyruvate dehydrogenase 8000U / L

[0064] Lactate dehydrogenase 10000U / L

[0065] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0066] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test subwavelength 405nm, volume ratio of manganese ion sample to reagent 1 and reagent 2 It is 2...

Embodiment 3

[0069] The manganese ion diagnosis / measurement reagent of the present embodiment is three reagents, comprises:

[0070] Reagent 1

[0071] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0072] Stabilizer 50mmol / L

[0073] Reduced coenzyme 0.25mmol / L

[0074] Oxaloacetate 12mmol / L

[0075] Acetyl-CoA 6mmol / L

[0076] Reagent 2

[0077] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0078] Stabilizer 500mmol / L

[0079] Pyruvate dehydrogenase 8000U / L

[0080] Lactate dehydrogenase 10000U / L

[0081] Reagent 3

[0082] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0083] Stabilizer 500mmol / L

[0084] Oxaloacetate decarboxylase 6000U / L

[0085] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0086] When measuring manganese ion concentration, set on the automatic biochemical analyzer: temperature 37°C, reaction t...

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Abstract

The invention relates to a manganese ion diagnosis / determination kit using the technology of an enzyme multiplication method, an enzyme colorimetric method and an ELISA method, a method for determining manganese ion concentration, and composition and components of a reagent, and belongs to the technical field of test and determination of medical science, food and environment. The kit comprises the following main components: buffer solution, reduced coenzyme, oxaloacetic acid, acetylcoenzyme A, oxaloacetic decarboxylase, pyruvate dehydrogenase, lactate dehydrogenase and a stabilizer. The method for determining manganese ion concentration comprises the following steps: mixing a sample and the reagent according to a certain volume ratio to perform a series of enzymic reaction, and then placing reactants under a UV / visible light analyzer to test the descending speed of absorbance at the position where the dominant wave length is 340nm to determine the concentration of manganese ion.

Description

technical field [0001] The invention relates to a manganese ion diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring manganese ion concentration, which belongs to the technical field of medicine / food / environmental inspection and determination. Background technique [0002] As a trace element, manganese is an indispensable and necessary component in the human body, and it is also a substance that is toxic to the human body. In the natural state, it exists in divalent, trivalent and tetravalent forms, and the lower the degree of oxidation, the higher the toxicity. There are generally eight different oxidation states of manganese. The biological significance of manganese is its diverse functions as a cofactor in the human body. However, in many enzyme activation reactions, Mn 2+ and Mg 2+ Can replace each other. [0003] Commonly used graphite furnace atomic absorption spectrophotometry, atomic emission ICP-OES. Neutron exc...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/25G01N33/84C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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