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Manganese ion diagnosis/determination reagent kit and method for determining manganese ion concentration

A technology of manganese ions and kits, applied in the field of medical/food/environmental inspection and determination

Inactive Publication Date: 2008-12-17
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Neutron excitation analysis (NAA) is the best method, but can only be determined in some well-conditioned laboratories

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0101] The manganese ion diagnosis / measurement reagent of the present embodiment is a single reagent, comprising:

[0102] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0103] Stabilizer 500mmol / L

[0104] Reduced coenzyme 0.25mmol / L

[0105] Ammonia ion 2mmol / L

[0106] Oxaloacetic acid 20mmol / L

[0107] Oxaloacetate decarboxylase 10000U / L

[0108] Alanine dehydrogenase 10000U / L

[0109] Glycine oxidase 10000U / L

[0110] Peroxidase 30000U / L

[0111] 4-amino antiarsenic 2mmol / L

[0112] Carbolic acid 10mmol / L

[0113] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0114] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 505nm, test sub-wavelength 600nm, the volume ratio of the manganese ion sample to the reagent to be tested is 1 / 25, and the reaction d...

Embodiment 2

[0117] The manganese ion diagnosis / measurement reagent of the present embodiment is a double reagent, comprising:

[0118] Reagent 1

[0119] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0120] Stabilizer 50mmol / L

[0121] Reduced coenzyme 0.25mmol / L

[0122] Ammonia ion 2mmol / L

[0123] Oxaloacetic acid 20mmol / L

[0124] 2,4,6-tribromo-3-hydroxy-benzenesulfonic acid 0.2mmol / L

[0125] Reagent 2

[0126] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0127] Stabilizer 500mmol / L

[0128] Oxaloacetate decarboxylase 10000U / L

[0129] Alanine dehydrogenase 10000U / L

[0130] Glycine oxidase 10000U / L

[0131] Peroxidase 25000U / L

[0132] 4-amino antiarsenic 0.6mmol / L

[0133] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0134] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavele...

Embodiment 3

[0137] The manganese ion diagnosis / fixing reagent of the present embodiment is three reagents, comprises:

[0138] Reagent 1

[0139] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0140] Stabilizer 50mmol / L

[0141] Reduced coenzyme 0.25mmol / L

[0142] Ammonia ion 2mmol / L

[0143] Oxaloacetic acid 20mmol / L

[0144] 3-methyl-2-benzothiazolone hydrazone 0.6mmol / L

[0145] Reagent 2

[0146] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0147] Stabilizer 500mmol / L

[0148] Alanine dehydrogenase 10000U / L

[0149] Glycine oxidase 10000U / L

[0150]Peroxidase 20000U / L

[0151] Dimethylaniline 2mmol / L

[0152] Reagent 3

[0153] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0154] Stabilizer 500mmol / L

[0155] Oxaloacetate decarboxylase 10000U / L

[0156] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0157] ...

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Abstract

The invention relates to a kit for diagnosing / mensurating manganese ions by utilizing the technologies of the enzymatic cycling amplification method, the enzymic colorimetric method and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the manganese ions, and belongs to the technology field of medical / food / environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, oxaloacetic acid, ammonia ions, oxaloacetic decarboxylase, alanine dehydrogenase, glycine oxidase, peroxidase, a reduced chromogen combination and a stabilizer. Through a series of enzymatic reactions, the colorless reduced chromogen combination is finally oxidized into the colored dyes, thereby mensurating the content of the dyes at 400nm-700nm of the wavelength by a visible light analyzer and further reflecting the concentration of the manganese ions directly.

Description

technical field [0001] The invention relates to a manganese ion diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring manganese ion concentration, which belongs to the technical field of medicine / food / environmental inspection and determination. Background technique [0002] As a trace element, manganese is an indispensable and necessary component in the human body, and it is also a substance that is toxic to the human body. In the natural state, it exists in divalent, trivalent and tetravalent forms, and the lower the degree of oxidation, the higher the toxicity. There are generally eight different oxidation states of manganese. The biological significance of manganese is its diverse functions as a cofactor in the human body. However, in many enzyme activation reactions, Mn 2+ and Mg 2+ Can replace each other. [0003] Commonly used graphite furnace atomic absorption spectrophotometry, atomic emission ICP-OES. Neutron exc...

Claims

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Application Information

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IPC IPC(8): G01N33/84G01N21/31C12Q1/26C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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