Method for rapidly detecting content of EGCG in tea polyphenol

A detection method and tea polyphenol technology, applied in the field of analytical chemistry, can solve the problems of high detection cost, large solvent consumption, long detection time, etc., and achieve the effects of strong elution ability, improved selectivity, and easy degassing

Pending Publication Date: 2019-08-23
广西轻工业科学技术研究院有限公司
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current detection method has many types of solvents, complex preparation, long detection time, fast flow rate, large solvent consumption, high detection cost, and is not suitable for routine detection and quality control.

Method used

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  • Method for rapidly detecting content of EGCG in tea polyphenol
  • Method for rapidly detecting content of EGCG in tea polyphenol
  • Method for rapidly detecting content of EGCG in tea polyphenol

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Embodiment 1

[0044] (1) Setting of ultra-high performance liquid chromatography conditions:

[0045] Using Agilent ZORBAX Eclipse Plus C 18 The chromatographic column (2.1×50 mm, 1.8 μm) uses methanol-1% acetic acid as the mobile phase, the flow rate is 0.55 mL / min, the column temperature is 35 °C, the detection wavelength is 276 nm, and the injection volume is 0.50 μL. The way of elution, the specific elution conditions are shown in the table below:

[0046]

[0047] (2) Preparation of reference substance solution: Precisely weigh EGCG, EC, EGC, GCG, ECG and caffeine reference substance 1.29, 1.02, 1.43, 1.10, 1.03, 0.25 mg respectively, add absolute ethanol to dissolve and dilute to 5mL, as the reference substance solution;

[0048] (3) Preparation of the test solution: Accurately weigh 4.14mg of tea polyphenols, add absolute ethanol to dissolve and dilute to 5mL, and use it as the test solution;

[0049] (4) System suitability test:

[0050] Take the test solution of tea polyphe...

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Abstract

The invention belongs to the field of analytical chemistry, and particularly relates to an ultra-high performance liquid chromatography rapid detection technology, in particular to a method for efficiently and rapidly detecting the content of EGCG in tea polyphenol as a raw material by utilizing the ultra-high performance liquid chromatography detection technology. A C18 chromatographic column isadopted; water-glacial acetic acid-methanol with a certain proportion is used as a mobile phase; the content of the EGCG in the tea polyphenol is rapidly detected in a gradient elution mode; and the theoretical plate number of the EGCG under the chromatographic condition is greater than 16,000, and the separation degree is greater than 3. By adopting the detection method, the EGCG, EC and caffeinerelatively difficult to separate are effectively separated; and the method is short in detection time, high in sensitivity, good in reproducibility, efficient and rapid, and can be applied to the EGCG content detection and quality control.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to an ultra-high performance liquid chromatography rapid detection technology, in particular to a method for efficiently and rapidly detecting EGCG content by using tea polyphenols as raw materials and utilizing the ultra high performance liquid chromatography detection technology. Background technique [0002] Tea polyphenols ( tea polyenohs, Tp ) is mainly composed of catechins, flavonoids, phenolic acids, and anthocyanins. while catechins ( catechins ) accounted for 60% to 80% of the total tea polyphenols. Modern research shows that catechin is not only an ideal natural antioxidant, but also a class of extremely valuable natural pharmaceutical raw materials. effect. The catechin monomer compounds in tea polyphenols mainly include epicatechin (EC), epigallocatechin (EGC), epicatechin gallate (ECG), gallocatechin gallate (GCG) ) and epigallocatechin gallate (EG...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/884
Inventor 曹敏王元春蒙秋艳冯德伟黄志钰韦丽婷程劲芝
Owner 广西轻工业科学技术研究院有限公司
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