A method and kit for inducing microglia from pluripotent stem cells

A technology of microglia cells and kits, applied in the field of stem cell biology, can solve the problems of few differentiation schemes, long time-consuming, difficult to control differentiation results, etc., and achieve the effect of reducing costs, reducing induction time, and simple methods
CN110205295BActive Publication Date: 2021-10-29INST OF ZOOLOGY CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF ZOOLOGY CHINESE ACAD OF SCI
Publication Date
2021-10-29

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Abstract

The invention discloses a method and a kit for inducing microglial cells from pluripotent stem cells. The present invention firstly provides a kit for inducing microglial cells. The present invention also provides a method for inducing microglial cells. The method and kit for inducing microglial cells of the present invention can successfully induce pluripotent stem cells into a large number of mature microglial cells with special morphology, expression of specific markers and good phagocytic function through primary hematopoietic progenitor cells. Co-cultivation technology and gene recombination technology, it only takes 35‑40 days to obtain mature microglia from pluripotent stem cells, reducing induction time and cost. The method is simple and convenient, making it possible to study the gene function of Key nodes in the development of glial cells and new therapeutic targets become possible, and also provide a basis for the construction of microglia with specific disease prevention and treatment functions, and provide a source for cell replacement therapy of microglia.
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Description

technical field

[0001] The invention relates to the field of stem cell biology, in particular to a method and a kit for inducing microglial cells from pluripotent stem cells. Background technique

[0002] Stem cells (Stem Cells) are a type of insufficiently differentiated cells that exist in multicellular organisms. Stem cells can self-renew through mitosis to produce more stem cells, and can also divide and differentiate to form a variety of specialized cells, tissues and organs. Induced pluripotent stem cell (iPSC) is a new type of stem cell obtained by transferring transcription factors or small molecular compounds into mammalian somatic cells, and usually has three germ layers similar to embryonic stem cells Differentiation ability, in theory, it can differentiate into all tissues and organs of the adult.

[0003] Induced pluripotent stem cells theoretically have the ability to differentiate into all types of specialized cells, and are very suitable for replacing cells...

Claims

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