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Method of detecting heparin or heparin-like substances in blood and kit

A detection method and heparin technology, applied in the field of heparin, can solve the problems of high detection cost, easy inactivation, high price, etc., and achieve the effects of low detection cost, easy storage and transportation, and stable biochemical properties

Inactive Publication Date: 2019-09-06
ZIRCON BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, thrombelastography is usually used to detect heparin in blood. Thromboelastography has the advantages of high sensitivity, fast speed and accuracy. However, heparinase must be used to decompose heparin in blood during detection. Heparinase is A very expensive substance, and because its essence is an enzyme, it is easy to inactivate. It must be stored in a freeze-dried state at low temperature during storage and transportation. It needs to be reconstituted into a liquid before use. This detection method is expensive and inconvenient to use

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Divide the same blood sample into two parts with the same mass (or volume) and add them to two test cups, which are respectively recorded as the first blood sample and the second blood sample. The volumes of the first blood sample and the second blood sample are both 0.34mL , then add 0.01mL of toluidine blue solution to the first blood sample to obtain a mixture; the preparation method of the toluidine blue solution is: add toluidine blue to the buffer mixture, and the buffer mixture comprises: Tris-Hcl buffer solution with a pH of 7.20 and Proclin300, the mass content of Proclin300 in the Tris-Hcl buffer solution is 0.2%; the mass content of toluidine blue in the buffer mixture is 0.1%.

[0059] Add 0.01 mL of thrombin solution to the above mixture and the second blood sample, the preparation method of the thrombin solution is: add thrombin to the buffer mixture, the buffer mixture includes: pH 7.20 Tris-Hcl buffer and Proclin300, the mass content of Proclin300 in the...

Embodiment 2

[0062] Divide the same blood sample into two parts with the same mass (or volume) and add them to two test cups, which are respectively recorded as the first blood sample and the second blood sample. The volumes of the first blood sample and the second blood sample are both 0.34mL , then add 0.01mL of protamine solution to the first blood sample to obtain a mixture; the preparation method of the protamine solution is: add protamine to the buffer mixture, and the buffer mixture comprises: Tris-Hcl buffer with a pH of 7.20 and Proclin300, the mass content of Proclin300 in the Tris-Hcl buffer is 0.2%; the mass content of protamine in the buffer mixture is 0.1%.

[0063] Add 0.02mL of calcium chloride solution to the above mixture and the second blood sample respectively, the preparation method of the calcium chloride solution is: add calcium chloride to the buffer mixture, the buffer mixture includes: pH 7.20 Tris-Hcl buffer and Proclin300, the mass content of Proclin300 in the T...

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Abstract

The invention provides a method of detecting heparin or heparin-like substances in blood, which comprises steps: the same blood sample is divided to two parts with the equal mass, and the two parts are recorded as a first blood sample and a second blood sample; the first blood sample is mixed with a heparin neutralizing agent to obtain a mixture; the mixture and a first coagulant are mixed, detection of a first coagulation time is carried out, and the first coagulation time is obtained; the second blood sample and a second coagulant are mixed, detection of a second coagulation time is carriedout, and the second coagulation time is obtained, wherein the first coagulant and the second coagulant have the same composition and the same use amount, and the first coagulation time and the secondcoagulation time have the same detection method; and if the time difference between the first coagulation time and the second coagulation time is greater than 5 seconds, heparin or heparin-like substances are thus contained in the blood sample. The method provided in the invention is suitable for clinical promotion, the detection cost is reduced, and the operation is easy.

Description

technical field [0001] The invention relates to the technical field of heparin, in particular to a detection method and kit for heparin or heparin-like substances in blood. Background technique [0002] At present, thrombelastography is usually used to detect heparin in blood. Thromboelastography has the advantages of high sensitivity, speed and accuracy. However, heparinase must be used to decompose heparin in blood during detection. Heparinase is It is a very expensive substance, and because its essence is an enzyme, it is easy to inactivate. It must be stored in a freeze-dried state at low temperature during storage and transportation, and it needs to be reconstituted into a liquid before use. This detection method is expensive and inconvenient to use. Contents of the invention [0003] In view of this, the object of the present invention is to provide a detection method for heparin or heparin-like substances in blood, the detection method provided by the present invent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/49
CPCG01N33/49G01N33/4905
Inventor 李钢李彤
Owner ZIRCON BIOTECH CO LTD
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