Purpose of survivin in preparation of colorectal cancer prognosis kit

A colorectal cancer and kit technology, applied in the field of colorectal cancer diagnosis and treatment, can solve the problem of no research report on the relationship between colorectal cancer and so on.

Inactive Publication Date: 2019-09-06
GUILIN MEDICAL UNIVERSITY
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no research report on the relationship between survivin expression and colorectal cancer

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Purpose of survivin in preparation of colorectal cancer prognosis kit
  • Purpose of survivin in preparation of colorectal cancer prognosis kit
  • Purpose of survivin in preparation of colorectal cancer prognosis kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Materials and methods

[0017] Patient and tissue samples. From 1984 to 2001, 142 cases of end-stage colorectal cancer (96 males and 46 females) were selected from the pathological archives of Hiroshima University Hospital, including 71 cases of lymph node metastasis and 25 cases of distant metastasis. The age of the patients ranged from 37 to 84 years old (mean, 63.8 years old). On histological examination, 52 cases were benign, 67 cases were moderate, and 23 cases were poorly differentiated colorectal cancer. For immunohistochemical examination, 4 μm serial sections were stained with hematoxylin and eosin for immunohistochemical analysis.

[0018] Immunohistochemical studies and staining evaluation.

[0019] After the antigen was repaired by microwave in citric acid buffer (pH 6.0), the slices were incubated overnight at 4°C with polyclonal anti-survivin antibody (NB500-201, Rofus Biologics, Littleton Co., Ltd. 1:1000 dilution), and passed Streptavidin-biotin-pero...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the field of diagnosis and treatment of colorectal cancer, in particular to the use of survivin in the diagnosis of colorectal cancer, thereby solving a problem of providing the purpose of survivin protein in the preparation of a colorectal cancer prognosis kit. The function of cytoplasmic survivin may play an important role in malignant tumor progression. The cytoplasm-nucleus solubility level and the colorectal cancer prognostic factor are more associated than the surviving total amount. The mode of suppressing positioning of survivin in the cytoplasm may serve as anovel way of suppressing the function of survivin in cancer therapy.

Description

technical field [0001] The invention relates to the field of diagnosis and treatment of colorectal cancer, in particular to the use of survivin in the diagnosis of colorectal cancer. Background technique [0002] Survivin is a member of the apoptosis inhibitory protein (apoptosis protein, IAP) family, which plays an important role in inhibiting apoptosis by inhibiting the activity of caspase. In addition, it is also a subunit of the chromosomal passenger complex (CPC), which includes subunits such as Aurora-b, Burn (endocentromere protein), and Borealin that regulate cell division. [0003] Survivin is unique in that it is expressed in fetal tissues and various human cancers, and some recent reports have shown that Survivin is expressed in specific adult tissues including colonic epithelium, normal endometrium, placenta and bone marrow. On the other hand, the intracellular localization of survivin in cancer cells has also been reported to express the biological characterist...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N33/57419G01N33/57446G01N33/57484G01N2800/52
Inventor 曾思恩齐广莹刘甜周立华谈建成
Owner GUILIN MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap