Extraction method of estrogen in North China leopard excrement
An extraction method and estrogen technology, applied in the field of hormone extraction, can solve the problems of large damage, animal stress, deviation, etc. of the North China leopard, and achieve the effects of reducing the operation difficulty coefficient, high extraction rate and high reliability
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Embodiment 1
[0026] A method for extracting estrogen in North China leopard feces, comprising the following steps:
[0027] (1) After thawing the frozen North China leopard feces sample, weigh 1g of North China leopard feces into a 30mL centrifuge tube, and add 10mL of ethanol solution with a mass concentration of 70% to the centrifuge tube;
[0028] (2) Place the centrifuge tube in a shaking water bath at 65°C for 15 minutes, then take the centrifuge tube out of the shaking water bath and cool it to 4°C, then put the centrifuge tube in a centrifuge and centrifuge at 3500r / min for 15 minutes, and take the supernatant to in a glass test tube;
[0029] (3) Add 5 mL of ethanol solution with a mass concentration of 70% to the lower sediment of the centrifuge tube to resuspend the lower sediment, vortex for 1 min, and then put the centrifuge tube into a centrifuge for 15 min at 3500 r / min, take The supernatant in the centrifuge tube is transferred to the glass test tube in step (2);
[0030] ...
Embodiment 2
[0033] A method for extracting estrogen in North China leopard feces, comprising the following steps:
[0034] (1) After thawing the frozen North China leopard feces sample, weigh 1g of North China leopard feces into a 30mL centrifuge tube, and add 10mL of 80% ethanol solution to the centrifuge tube;
[0035] (2) Place the centrifuge tube in a shaking water bath at 70°C for 15 minutes, then take the centrifuge tube out of the shaking water bath and cool it to 4°C, then put the centrifuge tube in a centrifuge at 4000r / min for 15 minutes, and take the supernatant to in a glass test tube;
[0036] (3) Add 5 mL of ethanol solution with a mass concentration of 80% to the lower sediment of the centrifuge tube to resuspend the lower sediment, vortex for 1 min, and then put the centrifuge tube into a centrifuge for 15 min at 4000 r / min, take The supernatant in the centrifuge tube is transferred to the glass test tube in step (2);
[0037] (4) Place the glass test tube containing the...
Embodiment 3
[0040] A method for extracting estrogen in North China leopard feces, comprising the following steps:
[0041] (1) After thawing the frozen North China leopard feces sample, weigh 1g of North China leopard feces into a 30mL centrifuge tube, and add 10mL of 80% ethanol solution to the centrifuge tube;
[0042] (2) Place the centrifuge tube in a shaking water bath at 70°C for 15 minutes, then take the centrifuge tube out of the shaking water bath and cool it to 4°C, then put the centrifuge tube in a centrifuge at 4000r / min for 15 minutes, and take the supernatant to in a glass test tube;
[0043] (3) Add 5 mL of ethanol solution with a mass concentration of 80% to the lower sediment of the centrifuge tube to resuspend the lower sediment, vortex for 1 min, place the centrifuge tube in a shaking water bath at 70°C for 15 min, and place the centrifuge tube Take it out from the shaking water bath and cool it down to 4°C, then put the centrifuge tube into a centrifuge at 4000r / min f...
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