Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Extraction method of estrogen in North China leopard excrement

An extraction method and estrogen technology, applied in the field of hormone extraction, can solve the problems of large damage, animal stress, deviation, etc. of the North China leopard, and achieve the effects of reducing the operation difficulty coefficient, high extraction rate and high reliability

Pending Publication Date: 2019-09-13
太原动物园 +1
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, blood sample analysis is usually used to measure the estrogen in the North China leopard. Due to the difficulty in taking blood from the North China leopard, it is necessary to capture, anesthetize or restrict the North China leopard. The damage is large, and the capture will cause stress to the animals, which will affect the estrogen level in the North China leopard. Therefore, the estrogen level measured by blood sample analysis may have a certain deviation from the true estrogen level in the North China leopard.
[0004] Feces are the natural metabolites of animals, which can truly reflect the changes in hormone levels in animals. Since collecting feces is relatively easy and does not harm animals, measuring the estrogen level in the feces of North China leopards will become a tool for studying its reproduction. An important direction, however, how to extract estrogen from the feces of the North China leopard has not been studied and reported

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for extracting estrogen in North China leopard feces, comprising the following steps:

[0027] (1) After thawing the frozen North China leopard feces sample, weigh 1g of North China leopard feces into a 30mL centrifuge tube, and add 10mL of ethanol solution with a mass concentration of 70% to the centrifuge tube;

[0028] (2) Place the centrifuge tube in a shaking water bath at 65°C for 15 minutes, then take the centrifuge tube out of the shaking water bath and cool it to 4°C, then put the centrifuge tube in a centrifuge and centrifuge at 3500r / min for 15 minutes, and take the supernatant to in a glass test tube;

[0029] (3) Add 5 mL of ethanol solution with a mass concentration of 70% to the lower sediment of the centrifuge tube to resuspend the lower sediment, vortex for 1 min, and then put the centrifuge tube into a centrifuge for 15 min at 3500 r / min, take The supernatant in the centrifuge tube is transferred to the glass test tube in step (2);

[0030] ...

Embodiment 2

[0033] A method for extracting estrogen in North China leopard feces, comprising the following steps:

[0034] (1) After thawing the frozen North China leopard feces sample, weigh 1g of North China leopard feces into a 30mL centrifuge tube, and add 10mL of 80% ethanol solution to the centrifuge tube;

[0035] (2) Place the centrifuge tube in a shaking water bath at 70°C for 15 minutes, then take the centrifuge tube out of the shaking water bath and cool it to 4°C, then put the centrifuge tube in a centrifuge at 4000r / min for 15 minutes, and take the supernatant to in a glass test tube;

[0036] (3) Add 5 mL of ethanol solution with a mass concentration of 80% to the lower sediment of the centrifuge tube to resuspend the lower sediment, vortex for 1 min, and then put the centrifuge tube into a centrifuge for 15 min at 4000 r / min, take The supernatant in the centrifuge tube is transferred to the glass test tube in step (2);

[0037] (4) Place the glass test tube containing the...

Embodiment 3

[0040] A method for extracting estrogen in North China leopard feces, comprising the following steps:

[0041] (1) After thawing the frozen North China leopard feces sample, weigh 1g of North China leopard feces into a 30mL centrifuge tube, and add 10mL of 80% ethanol solution to the centrifuge tube;

[0042] (2) Place the centrifuge tube in a shaking water bath at 70°C for 15 minutes, then take the centrifuge tube out of the shaking water bath and cool it to 4°C, then put the centrifuge tube in a centrifuge at 4000r / min for 15 minutes, and take the supernatant to in a glass test tube;

[0043] (3) Add 5 mL of ethanol solution with a mass concentration of 80% to the lower sediment of the centrifuge tube to resuspend the lower sediment, vortex for 1 min, place the centrifuge tube in a shaking water bath at 70°C for 15 min, and place the centrifuge tube Take it out from the shaking water bath and cool it down to 4°C, then put the centrifuge tube into a centrifuge at 4000r / min f...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of hormone extraction methods, and particularly relates to an extraction method of estrogen in North China leopard excrement. The method comprises the following steps of weighing 1 g of unfrozen North China leopard excrement in a centrifuge tube, and adding 10 ml of an ethanol solution with the mass concentration of 80%; placing in oscillating water bath at 70 DEG C for 15 minutes, cooling to 4 DEG C, putting into a centrifuge for centrifuging at 4000 r / min for 15 minutes, and taking supernate; adding 5 mL of an ethanol solution with the mass concentration of 80% into lower precipitate, carrying out vortex oscillation for 1 minute, putting into the centrifuge for centrifuging at 4000 r / min for 15 minutes, and merging the supernate; carrying outwater bath air drying on the merged supernate at the temperature of 60 DEG C; and adding PB for redissolving, and storing at -20 DEG C after redissolving is complete. The method is easy to operate andhigh in estrogen extraction rate, and has no damage to the North China leopard, and stress reaction of the North China leopard cannot be generated during an extraction process, so that a detection result is high in reliability.

Description

technical field [0001] The invention belongs to the technical field of hormone extraction methods, in particular to a method for extracting estrogen from the feces of North China leopards. Background technique [0002] The North China leopard is a subspecies of the leopard, belonging to the class Mammalia, order Carnivora, family Felidae, and large carnivores. In recent years, due to habitat destruction and other reasons, its number has decreased sharply, and it has been listed as a national first-level key protected animal; therefore, the reproduction of the North China leopard has attracted widespread attention from the industry. [0003] The estrogen level in the North China leopard has a very important effect on its reproduction, so it is necessary to carry out research on the estrogen in the North China leopard. At present, blood sample analysis is usually used to measure the estrogen in the North China leopard. Due to the difficulty in taking blood from the North Chin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N1/34G01N1/40
CPCG01N1/34G01N1/4055G01N1/4077
Inventor 李亮亮由玉岩张卓孙乐天金修哲卫泽珍杨引亮刘学锋张成林李晓光
Owner 太原动物园
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products