Method for identifying queshan black pig

An identification method and technology for black pigs, applied in biochemical equipment and methods, microbiological determination/testing, DNA/RNA fragments, etc., can solve the problem of similar morphological characteristics of black pig breeds

Pending Publication Date: 2019-09-17
HENAN AGRICULTURAL UNIVERSITY +1
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  • Application Information

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Problems solved by technology

[0006] The purpose of this application is to provide a method for identifying Queshan black pigs by using the mutation sites in the mitochondrial D-loop region, so as to better solve the problem that the morphological characteristics of black pig breeds

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  • Method for identifying queshan black pig
  • Method for identifying queshan black pig
  • Method for identifying queshan black pig

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Experimental program
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Embodiment 1

[0049] In existing studies, the mitochondrial DNA structure of mammals is simple, stable, and follows maternal inheritance. It is often used as a genetic marker to study the origin, evolution, kinship, and population genetic structure of domestic animals. Based on this feature, the inventor amplified and compared the mitochondrial genomes of four local pigs in Henan Province (Yuxi black pig, Nanyang black pig, and Huainan pig) and found that at position 185 of the gene in the D-loop region , the Queshan black pig has a unique mutation g.185T mutation to C; in addition, at the 589th position of the D-loop region gene, the Queshan black pig has an inserted base G. Further experimental verification was carried out. The specific process is described as follows.

[0050] (1) Primer design

[0051] The inventor selected the pig D-loop region gene in NCBI (accession number: NC_000845.1), and designed a PCR primer for the upstream and downstream of the 185th base of the D-loop regio...

Embodiment 2

[0071] On the basis of Example 1, in order to verify the specificity and reliability of the mutation site, the inventor further downloaded nine other Chinese local pig breeds (Huaihua pig, Taoyuan pig, Laiwu pig, Jinhua pig, Jinhua pig) from NCBI. pigs, Meishan pigs, Wuzhishan pigs, Luchuan pigs, Min pigs, and Erhualian), three major Western commercial pig breeds (Duroc, Yorkshire, and Landrace pigs), two Southeast Asian local pig breeds (Moncai pigs, JNP pigs ) to compare the sequences one by one, the specific comparison results are as follows image 3 , Figure 4 shown. It can be seen from the figure that the D-loop regions g.185C and g.589G of Queshan black pigs are indeed breed-specific and can be used to identify Queshan black pigs.

[0072] It should be noted that, in NCBI, the specific accession numbers of the D-loop area of ​​each pig breed: Huai pig MG250536.1, Taoyuan pig AM040653.1, Laiwu pig EF590174.1, Jinhua pig AB041477.1, Meishan pig AM040647.1, Wuzhishan pi...

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Abstract

The invention belongs to the technical field of animal variety identification, particularly relates to a method for identifying a queshan black pig by utilizing a D-loop area mutation site. The method comprises the steps of extraction of a genome DNA of a to-be-identified pig variety sample, PCR amplification, comparison identification and the like. During specific identification, if the 185 base is C or the 589 base is G, a to-be-identified pig variety is a queshan black pig variety having a genetic relationship with the queshan black pig. The use of the identification method has better practical value for variety protection, variety breeding and market development of the queshan black pig. At the same time, compared with a large-scale sequencing method, the identification method has the advantages that the operation is simple, rapid and efficient, and the cost is low, and therefore, the identification provides better technical reference for protection identification, variety breeding and market development of other local pig varieties, and has better popularization and application significance.

Description

technical field [0001] The application belongs to the technical field of animal variety identification, and in particular relates to a method for identifying Queshan black pigs by utilizing mutation sites in the D-loop region. Background technique [0002] Queshan black pig is a local pig breed. Its main production area is in Queshan County, Henan Province. Due to the inconvenient transportation in the production area, it has formed a natural isolation. After long-term selection and breeding by the local working people, it has gradually formed a pig breed that adapts to the local breeding environment. A local excellent variety has been formed. Because of its tender meat, dark red color, rich marbling, strong fecundity, strong adaptability, docile temperament, and easy training, it is one of the excellent genetic resources of livestock and poultry in my country. [0003] One of the preconditions for the selection and cultivation of excellent pig breeds is the accurate identi...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 李新建李秀领薛亚辉黄志伟贺萌萌
Owner HENAN AGRICULTURAL UNIVERSITY
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