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Extract for inhibiting liver cancer cells and in saussurea involucrata culture and preparation thereof for treating liver cancer

A technology of Saussurea sauraceae culture and inhibition of liver cancer, which is applied in the direction of drug combination, plant raw materials, medical preparations containing active ingredients, etc., can solve the problems of long drug cycle, achieve short drug cycle, excellent inhibitory effect, and good inhibitory effect Effect

Inactive Publication Date: 2019-09-20
DALIAN PRACTICAL BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, there are many preparations for the treatment of liver cancer. Although they can play a certain role in inhibiting liver cancer cells, they all have problems such as long drug cycles.

Method used

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  • Extract for inhibiting liver cancer cells and in saussurea involucrata culture and preparation thereof for treating liver cancer
  • Extract for inhibiting liver cancer cells and in saussurea involucrata culture and preparation thereof for treating liver cancer
  • Extract for inhibiting liver cancer cells and in saussurea involucrata culture and preparation thereof for treating liver cancer

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Embodiment Construction

[0030] The present invention will be further explained through specific experimental cases below, but it is not intended to limit the protection scope of the present invention.

[0031] 1.1 Instruments and reagents

[0032] Olympus CX22 fluorescent inverted microscope was used to observe the state of cells, Shanghai flash microplate reader.

[0033] The culture of snow lotus was provided by Dalian Puruikang Biotechnology Co., Ltd.; the liver cancer Hep3B cell line was purchased from Shenyang Dingguo Bioreagent Company, and the liver cancer HepG2 cell line was subcultured by the laboratory of Liaoning University of Traditional Chinese Medicine; DMEM medium (Gibico), calf Serum (Gibico) and double antibody (Gibico) MTT (Sigma) were purchased from Shenyang Dingguo Biological Reagent Company; all cell culture instruments used in the experiment were sterile products.

[0034] Petroleum ether (30-60), chloroform, ethyl acetate, and n-butanol were all of analytical grade and purchas...

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Abstract

The invention discloses an extract for inhibiting liver cancer cells and in a saussurea involucrata culture and a preparation thereof for treating liver cancer. The extract is obtained by taking the saussurea involucrata culture as a raw material and chloroform or n-butanol as an organic solvent for backflow extraction. It is accidentally found for the first time that the extract extracted by taking chloroform or n-butanol as the organic solvent has excellent inhibiting effect on HepG2 cells and Hep3B cells of liver cancer and is short in medication cycle, and the problem that existing preparations for treating the liver cancer are long in medication cycle can be solved effectively. The preparation contains chloroform extract or n-butanol extract, thereby having the advantages of excellent liver cancer cell inhibiting effect and short medication cycle.

Description

technical field [0001] The present invention relates to the technical field of modern medicine, and in particular to an extract from Saussurea chinensis culture that inhibits liver cancer cells and a preparation for treating liver cancer. Background technique [0002] Liver cancer is one of the most common malignant tumors in our country. Since there are no obvious symptoms in the early stage of liver cancer, when the symptoms appear, most of them are already in the advanced stage, which leads to the delay of the disease. [0003] At present, there are many preparations for the treatment of liver cancer. Although they can play a certain role in inhibiting liver cancer cells, they all have problems such as long drug cycles. [0004] Therefore, how to develop a preparation that can not only achieve the effect of inhibiting liver cancer cells, but also shorten the drug cycle has become an urgent problem to be solved. Contents of the invention [0005] In view of this, the pr...

Claims

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Application Information

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IPC IPC(8): A61K36/285A61P35/00
CPCA61K36/285A61K2236/35A61K2236/39A61P35/00
Inventor 刘汉石孙晓琳赵晓玫
Owner DALIAN PRACTICAL BIOTECH
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