Method for knocking out PD-L1 gene in keratinocytes by CRISPR/Cas9 gene editing method

A PD-L1 and gene editing technology, applied to other methods of inserting foreign genetic material, cells modified by introducing foreign genetic material, animal cells, etc., can solve the problems of psoriasis patients who are difficult to continue to adhere to treatment and adverse reactions.
CN110257376APending Publication Date: 2019-09-20SHANGHAI CHANGHAI HOSPITAL
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI CHANGHAI HOSPITAL
Publication Date
2019-09-20

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Abstract

The invention relates to the field of cell model construction, and concretely relates to a method for knocking out a PD-L1 gene in keratinocytes by a CRISPR / Cas9 gene editing method, and a keratinocyte model constructed by the method and an application thereof. The method has the advantages that the PD-L1 knockout keratinocyte stable-transfection strain constructed by the invention can be used not only for the research of psoriasis, but also for the research of other skin diseases with low expression of PD-L1. The construction of the PD-L1 knockout cell strain is helpful for exploring the role of PD-L1 in skin-related diseases such as psoriasis, pityriasis rosea, and allergic contact dermatitis, and further explores the function of PD-L1 in keratinocytes.
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Description

technical field

[0001] The invention relates to the field of cell model construction, specifically, a CRISPR / Cas9 gene editing method for knocking out the PD-L1 gene in keratinocytes, and the keratinocyte model constructed by the method and its application. Background technique

[0002] Psoriasis is a genetically related, immune-mediated chronic inflammatory disease, usually with skin and joint lesions. The clinical manifestations of psoriasis are red plaques with clear boundaries on the skin surface, accompanied by silvery white dry scales, often accompanied by pruritus symptoms, and easy to relapse, which brings great physiological, psychological and economic burdens to patients.

[0003] Skin consists of epidermis (including stratum corneum, granular layer, spinous layer and basal layer), dermis (papillary layer and reticular layer) and subcutaneous tissue. The main component of the epidermis is keratinocytes, which play an important role in the skin's immune response. ...

Claims

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