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Warm-blooded animal genome DNA extraction equipment

A warm-blooded animal and extraction equipment technology, which is applied in the field of DNA extraction, can solve the problems of high price and cumbersome steps of the automatic magnetic bead DNA extraction instrument, and achieve the effect of low equipment cost and simple operation

Inactive Publication Date: 2019-09-27
临海永存智能技术有限公司
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Problems solved by technology

[0003] Technical issues: At present, magnetic bead method is commonly used in DNA extraction equipment, and the operation steps of magnetic bead method to extract DNA are cumbersome, and the automatic magnetic bead method DNA extractor is expensive

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  • Warm-blooded animal genome DNA extraction equipment
  • Warm-blooded animal genome DNA extraction equipment
  • Warm-blooded animal genome DNA extraction equipment

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Embodiment Construction

[0027] Combine below Figure 1 to Figure 12 The present invention is described in detail, and for convenience of description, the orientations mentioned below are now stipulated as follows: figure 1 The up, down, left, right, front and back directions of the projection relationship itself are the same.

[0028] The present invention relates to a warm-blooded animal genome DNA extraction equipment, which is mainly used for DNA extraction. The present invention will be further described below in conjunction with the accompanying drawings of the present invention:

[0029]A warm-blooded animal genomic DNA extraction device according to the present invention includes an extraction box 11, an extraction chamber 12 with an opening facing forward is provided in the extraction chamber 12, and a centrifugal device for rotating and centrifuging is provided in the extraction chamber 12 101, the extraction chamber 12 is provided with a mobile device 102 located on the right side of the c...

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Abstract

The invention discloses warm-blooded animal genome DNA (deoxyribonucleic acid) extraction equipment which comprises an extraction tank, wherein an extraction cavity with an opening facing forward is arranged in the extraction tank; a centrifuge device for rotary centrifugation is arranged in the extraction cavity; a moving device located on the right side of the centrifuge device is arranged in the extraction cavity and used for providing ascending, descending and back and forth movement; a liquid extraction device located on the left side of the centrifuge device is arranged in the extraction cavity; and an injection device located on the upper sides of the moving device and the liquid extraction device is arranged in the extraction cavity. The extraction equipment performs DNA by a classical phenol-chloroform method; and a spherical valve body in the injection device can automatically switch injection pipelines, so that different solutions can be injected into tubes filled with animal tissues. Therefore, the equipment can automatically achieve the DNA extraction by the phenol-chloroform method; and as compared with a magnetic bead, the equipment is simple to operate, adopts a common mechanical structure and is lower in cost.

Description

technical field [0001] The invention relates to the technical field of DNA extraction, in particular to a warm-blooded animal genome DNA extraction device. Background technique [0002] The classic method of DNA extraction is the phenol-chloroform method. First, use protease K and SDS to break the cells, digest the protein, and add an equal volume of phenol-chloroform mixture after the cells are lysed. According to the characteristics that DNA is easily soluble in water and not easily soluble in organic solvents , in an organic solvent environment, after the protein is denatured, precipitated and centrifuged, the organic solvent exists in the bottom layer of the test tube, the DNA exists in the upper layer of water, and the protein precipitate exists between the two liquids, and then the DNA in the water is extracted with a phenol-chlorine solution. After centrifugation, the supernatant is taken to realize DNA extraction, but the current DNA extraction equipment generally ad...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/04
CPCC12N15/1003
Inventor 叶吉利
Owner 临海永存智能技术有限公司
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