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Detection method and application of metabolic markers in biological sample

A technology of metabolic markers and biological samples, which is applied in the application field of Xiaoyao San metabolic markers and its detection, and the preparation of depression drug efficacy evaluation kits, which can solve the problems of modernization and lack of traditional Chinese medicine prescriptions in Xiaoyao San

Inactive Publication Date: 2019-09-27
SHANXI UNIV
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Problems solved by technology

However, Xiaoyao San lacks an objective pharmacodynamic evaluation system that reflects the overall characteristics of the prescription in the treatment of depression. This problem is a key scientific issue that plagues the modernization of Xiaoyao San's traditional Chinese medicine prescriptions, and is also a bottleneck for the internationalization of this traditional Chinese medicine.

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  • Detection method and application of metabolic markers in biological sample
  • Detection method and application of metabolic markers in biological sample
  • Detection method and application of metabolic markers in biological sample

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Embodiment Construction

[0033] (1) Data collection

[0034] Patients with early depression in the Mental Health Department of the First Affiliated Hospital of Shanxi Medical University.

[0035] Screening of enrolled cases: Patients diagnosed with single or repeated depressive episodes according to the diagnostic criteria of "Chinese Classification and Diagnostic Criteria of Mental Diseases" (CCMD-3); 17 points < HAMD17 score < 24 points at baseline, age 18 to 65 years old; male or female, outpatient or inpatient; depressive symptoms for at least 1 month.

[0036] Xiaoyao San prescription: Bupleurum, Angelica, Paeoniae Alba, fried Atractylodes macrocephala, Poria cocos 60g each, roasted licorice 30g, ginger, mint 20g each. One dose per day, twice in the morning and evening, 180 mL each time, equivalent to 1.0 g crude drug per mL, for 8 weeks.

[0037] In this embodiment, patients with depression were taken as subjects, and they were randomly divided into a test group and a control group. From the ...

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Abstract

The invention belongs to the technical field of metabonomics, and provides Xiaoyao San metabolic markers in a biological sample, and a detection method and an application thereof. A pharmacological action mechanism of Xiaoyao San for treating depression is defined. The metabolic markers are 4-oxo hex-2-ene dioic acid, creatinine, L-cystathionine and acetylphosphate. A UPLC-Q-TOF / MS technology is adopted for analyzing the plasma and metabolic profile change of patients in a drug administration group and a control group of the Xiaoyao San, and then a multivariate statistical analysis method and an orthogonal partial least square discriminant analysis (OPLS-DA) method are adopted for screening the changes of the metabolic markers of the Xiaoyao San after 8-week treatment. The study is used as a first clinical metabonomics study of the Xiaoyao San; the influence of the metabolic level of the Xiaoyao San is found; the action mechanism of the Xiaoyao San is preliminarily illuminated in metabolic pathway; and a new method is provided for predicting the pharmacological mechanism of the Xiaoyao San for treating the depression.

Description

technical field [0001] The invention belongs to the technical field of metabolomics, and relates to a detection method and application of metabolic markers in biological samples, specifically Xiaoyao San metabolic markers in biological samples, a detection method and application thereof, and the metabolic markers are used in the preparation of depression drug curative effect Use of the evaluation kit. Background technique [0002] Metabolomics is a rapidly developing omics technology following genomics, transcriptomics and proteomics, and is an important part of systems biology. Integrality is the basic point of view of metabolomics theory, which mainly uses nuclear magnetic resonance, mass spectrometry and other technical means to study the changes and laws of metabolites after biological systems are stimulated or disturbed. Changes in the levels of these metabolites are closely related to related metabolic pathways. By studying changes in metabolite levels and related met...

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Application Information

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IPC IPC(8): G01N30/88G01N33/487
CPCG01N30/88G01N33/48707G01N33/48792G01N2030/8822
Inventor 秦雪梅高耀田俊生令狐婷武嫣斐
Owner SHANXI UNIV
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