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Method for preventing and controlling adult willow plagiodera versicolora laicharting and avoidance agent used by method

A repellent and blue-leaf technology, applied in the fields of pest control, animal repellent, botanical equipment and methods, etc., can solve the problems of low pertinence, human health threat, low efficiency of manual killing, etc., and achieve good economic benefits. and social benefits, good practical application value, and obvious avoidance effect

Active Publication Date: 2019-10-11
句容市空凤来仪生态农业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the generally applied broad-spectrum insecticides are costly and less targeted, and may even lead to negative effects due to the reduction of natural enemies of Leaf beetle, and long-term use is likely to make insects resistant
Salix plants are widely used in landscaping. The insect mainly occurs in parks and scenic areas. The use of broad-spectrum insecticides will damage the environment and pose a threat to human health
At the same time, the efficiency of manual hunting is low and it is difficult to apply it in actual production practice

Method used

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  • Method for preventing and controlling adult willow plagiodera versicolora laicharting and avoidance agent used by method
  • Method for preventing and controlling adult willow plagiodera versicolora laicharting and avoidance agent used by method
  • Method for preventing and controlling adult willow plagiodera versicolora laicharting and avoidance agent used by method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Pick 5g each of fresh willow leaves and willow leaves, and place them in 50mL extraction bottles for extraction. The specific operation process is as follows: the top of the bottle is sealed with plastic wrap, and the solid phase microextraction head 65 μm PDMS / DVB manual solid phase microextraction head (Supelco) is used for adsorption. The extraction bottle is placed in a constant temperature water bath at 40°C for 40 minutes. The solid-phase microextraction head was treated at 250°C for 3 minutes for desorption treatment, and the obtained substances were analyzed by GC-MS respectively. Analysis results such as figure 1 shown. Depend on figure 1 It can be seen that the retention time of the difference substance is 10.17min. By comparing the compound database, the compound represented by this peak is trans-3-hexenol.

Embodiment 2

[0026] Under the condition of not feeding on leaves, the selectivity determination of the adults of the willow leaf beetle to the repellant and the control:

[0027] The concentration of the paraffin oil solution of the repellant trans-3-hexenol includes 0.001, 0.01, 0.1 and 1 mol / L.

[0028] The preparation method of the above solution: first prepare 1mol / L paraffin oil solution of trans-3-hexenol: add 5mL of trans-3-hexenol into an empty 50mL centrifuge tube, dilute to 50mL, shake and mix well . Then use a pipette gun to take out 1mL, 0.1mL and 0.01mL of the prepared 1mol / L paraffin oil solution of trans-3-hexenol respectively and add them to the above-mentioned empty centrifuge tube, and set the volume to 10mL to prepare 0.1, 0.01 and 0.001mol / L paraffin oil solution of trans-3-hexenol.

[0029] test methods:

[0030] 1. The long arm of the Y-shaped olfactometer is 10cm long, the two short arms are 6cm long, the angle between the two arms is 75°, the diameter is 1cm, and...

Embodiment 3

[0036] Determination of the selectivity of the adults of the willow leaf beetle to the repellant and the control under the condition of feeding on leaves

[0037] Salix indigo beetle eats the leaves of Dustpan willow seriously. Compared with Example 1, in this example, the leaves of Dustpan willow that Salix indigo beetle likes to eat were added to the repellant and the control group, and the selectivity was determined.

[0038] The concentrations of repellants used include: 0.001, 0.01, 0.1 and 1 mol / L trans-3-hexenol in paraffin oil.

[0039] The preparation method of the above solution: first prepare 1mol / L trans-3-hexenol paraffin oil solution: add 5mL trans-3-hexenol to an empty 50mL centrifuge tube, set the volume to 50mL, shake and mix uniform. Then use a pipette gun to take out 1mL, 0.1mL and 0.01mL of the prepared 1mol / L paraffin oil solution of trans-3-hexenol and add them to the above-mentioned empty centrifuge tube, and make the volume to 10mL to make 0.1mol / L , ...

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Abstract

The invention discloses a method for preventing and controlling adult willow plagiodera versicolora laicharting and an avoidance agent used by the method, and belongs to the technical field of insectavoidance agents. According to the method for preventing and controlling the adult willow plagiodera versicolora laicharting, trans-3-hexenol or a solution of the trans-3-hexenol is applied on willows. A solid-phase micro-extraction head is adopted for extracting the trans-3-hexenol from leaves of salix triandra, and the preparation has a long release period, is simple to prepare, and has an obvious avoidance effect on the adult willow plagiodera versicolora laicharting. The functional component trans-3-hexenol in the avoidance agent exists in some plants in nature, has no harm to people and livestock, is environmentally friendly, and has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of insect repellents, and in particular relates to a method for preventing and controlling adult beetle beetle and the repellent used. Background technique [0002] Willow is the most widely used tree species in urban and rural areas and landscaping in my country. Plagiodera versicolora is a leaf-feeding pest that has the widest range of damage and the most serious damage to Salix species. The willow blue leaf beetle belongs to the genus Plagiodera of the family Chrysomelidae in the order Coleoptera. It feeds on the leaves and shoots of willow trees. Most species of the genus Salix are affected by it, including willow willow, willow willow and weeping willow, etc. . In the southern provinces of my country, the insect pest occurs for 8-9 generations every year, and when the damage is serious, it can cause the death of seedlings, which is very harmful to the cultivation and production of willow seedlings. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N31/02C07C29/76A01G13/00C07C33/025A01P17/00
CPCA01G13/00A01N31/02C07C29/76C07C33/025
Inventor 尹佟明凌嘉昊李小平李淑娴
Owner 句容市空凤来仪生态农业有限公司
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