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Hippocampal circRNA sequencing analysis for schizophrenia mouse model and kit

A technology for schizophrenia and mouse models, applied in DNA/RNA fragments, recombinant DNA technology, microbial measurement/inspection, etc., can solve problems such as difficult collection and achieve the effect of improving accuracy

Inactive Publication Date: 2019-10-11
中国人民解放军联勤保障部队第九0四医院
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Problems solved by technology

However, subject to the limitations of bioethics, it is difficult to collect living brain tissue based on human schizophrenia. Recently, the neurodevelopmental mouse model of schizophrenia has attracted the attention of researchers. Currently, there are modeling standards, modeling procedures and Detection Indicator,

Method used

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  • Hippocampal circRNA sequencing analysis for schizophrenia mouse model and kit
  • Hippocampal circRNA sequencing analysis for schizophrenia mouse model and kit
  • Hippocampal circRNA sequencing analysis for schizophrenia mouse model and kit

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Embodiment Construction

[0029] A hippocampal biomarker in a neurodevelopmental mouse model of schizophrenia, wherein the marker is circRNA2.

[0030] A method for detecting hippocampal biomarkers in a neurodevelopmental mouse model of schizophrenia, comprising the following steps:

[0031] Brain tissue collection, three groups of mice in the control group (NC), schizophrenia model group (SZ), and treatment group (Treated) were decapitated at 100 days and the whole brain was removed;

[0032] RNA extraction and library construction to extract the total RNA of the sample, and use the kit ribo-zero to digest the ribosomal RNA; add the interrupting reagent to interrupt the RNA into short fragments, use the interrupted RNA as a template, and use six base random Primers are used to synthesize one-strand cDNA, and then a two-strand synthesis reaction system is prepared to synthesize two-strand cDNA. During cDNA second-strand synthesis, dUTP is used instead of dTTP, and then different adapters are connected,...

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Abstract

The invention belongs to the technical field of biology, and relates to a hippocampal biomarker for a schizophrenia neurodevelopment mouse model, a detection method and a kit. The invention provides the hippocampal biomarker circRNA2 for the schizophrenia neurodevelopment mouse model, the detection method of the marker and the kit for the content of the marker. The invention further provides a biomarker circRNA2 for the hippocampus intervention of the schizophrenia neurodevelopment mouse model and a detection method and kit of the marker. The invention also provides a circRNA-signal pathway-target gene network map of the circRNA2 and a GO / KEGG enrichment analysis result of differential circRNA2 in the hippocampus. The hippocampal biomarker, the detection method and the kit have the advantages that the sample model is established to prove that schizophrenia can be caused to mice due to pregnancy neurological injuries and post-natal stress stimulation; it is discovered for the first timethat the hippocampal circRNA of the mice has difference in expression level, and the change can be reversed by olanzapine intervention. Great significance is provided for studying a relationship between the onset of schizophrenia and epigenetics and searching for new drugs for schizophrenia.

Description

technical field [0001] The invention relates to the field of biotechnology, and relates to the technical field of hippocampal biomarkers, detection methods and kits of schizophrenia neurodevelopmental mouse models, in particular to hippocampal biomarkers and detection methods of schizophrenia neurodevelopmental mouse models. Background technique [0002] Schizophrenia (SZ) is a serious mental illness characterized by high relapse rate, high disability rate, and chronic persistence, which seriously affects individuals, families, and society. In 2008, McGrath et al. collected the epidemiological data of schizophrenia around the world with very conservative diagnostic criteria, showing that its lifetime prevalence (lifetime prevalence), annual average incidence rate and lifetime morbidity rate (lifetime morbid risk) were 0.46%, 0.015% and 0.72%, and there is an increasing trend year by year. If the diagnostic criteria are relaxed, the incidence will increase by 1-2 times. The...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12N15/113
CPCC12Q1/6883C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 张理义
Owner 中国人民解放军联勤保障部队第九0四医院
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