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Method for simultaneously measuring contents of four active ingredients in fleabane flowers

A technology of active ingredients and scutellaria, which is applied in the field of simultaneous determination of the contents of four active ingredients in scutellaria breviscapus, can solve the problems that there is no relationship between scutellaria breviscapus reported in literature, and the quality of scutellaria breviscapus cannot be effectively proved, so as to achieve good quality monitoring, quality evaluation, Exclusive performance and good effect

Active Publication Date: 2019-10-18
YUNNAN AGRICULTURAL UNIVERSITY
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Problems solved by technology

Literature research has found that there are significant differences in the flavonoids and total flavonoids content of scutellaria breviscapus in different regions, which poses a great challenge to the quality control of scutellaria breviscapus and the selection of special varieties for pharmaceutical companies. The relationship between total flavonoid content
The current Chinese Pharmacopoeia controls the composition of breviscapine, mainly through the determination of extracts and the content of scutellarin by high-performance liquid chromatography, which cannot effectively prove the quality of breviscapine, and further research is needed

Method used

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  • Method for simultaneously measuring contents of four active ingredients in fleabane flowers
  • Method for simultaneously measuring contents of four active ingredients in fleabane flowers
  • Method for simultaneously measuring contents of four active ingredients in fleabane flowers

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Embodiment

[0032] A method for simultaneously determining the content of four active ingredients in Breviscapus breviscapus

[0033] (1) preparation of standard product solution; the preparation of described standard product solution comprises the following steps: accurately take by weighing 4 standard products of scutellarin, scutellarin, scutellarein, and apigenin an appropriate amount, place them in a 10ml volumetric flask Dissolve in methanol and dilute to volume, shake well to prepare standard solutions with concentrations of 660 μg / ml, 673 μg / ml, 633 μg / ml, and 700 μg / ml, and store at 4°C for later use.

[0034] (2) preparation of need testing solution; the preparation of described need testing solution comprises the following steps: get scutellaria breviscapus sample, extract with ethanol, filter, obtain extract, filter with the microporous membrane of 0.22 μm again, get filtrate ,spare. Wherein, ethanol extraction is carried out with an ethanol solution with a volume concentrati...

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Abstract

The invention discloses a method for simultaneously measuring contents of four active ingredients in fleabane flowers. The Four active ingredients include scutellarin, apigenin-7-o-glucronide, scutellarein and apigenin. According to the method, the chromatographic column employs a C18 liquid chromatographic column; the flow rate is 0.8 to 1.2ml / min; the column temperate is 25 DEG C to 35 DEG C; the detection wavelength is 335nm; the sample injection amount is 2 microliters; the mobile phase A is water; the mobile phase B is acetonitrile; the mobile phase C is 0.3% of phosphoric acid aqueous solution; and a gradient elution mode is employed. The method has advantages of high accuracy and reliability and the good scientific and reasonable effect.

Description

technical field [0001] The invention belongs to the technical field of component analysis of traditional Chinese medicines, in particular to a method for simultaneously determining the contents of four active components in breviscapus breviscapus. Background technique [0002] Erigeron breviscapus (Vant.) Hand-Mazz.] is the whole herb of Compositae, also known as Erigeron breviscapus (Vant.) Hand-Mazz. It is mainly distributed in Yunnan, Guizhou, Sichuan and Guangxi in southwest my country. , especially in Yunnan, accounting for about 98%. Erigeron breviscapus was first recorded in "Southern Yunnan Materia Medica", and it was also included in a part of "Chinese Pharmacopoeia". Erigeron breviscapus contains more than 50 kinds of chemical components such as flavonoids, pyrones, and sesquiterpenes, which have the functions of promoting blood circulation, relaxing meridians, relieving pain and eliminating accumulation. The main medicinal active ingredients in Erigeron breviscapu...

Claims

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Application Information

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IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8813
Inventor 张广辉陈庚杨生超刘冠泽赵艳段绍凤李莹闫静龚建溪郭兆宽
Owner YUNNAN AGRICULTURAL UNIVERSITY
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