Fluorescence Spectrum Detection System Based on Negative Feedback Regulation

Abstract

The invention discloses a fluorescence spectrum detection system based on negative feedback adjustment, which includes: a lower computer, a light source, an excitation monochromator, a beam splitter, a photomultiplier tube at a monitoring end, an amplification circuit, a grating, a fluorescence detection area, and an emission monochromator. The lower computer controls the light source to emit laser light, and after being screened by the excitation monochromator, the same two paths of light are formed in the beam splitter, and one path of light passes through the photomultiplier tube and the amplifier circuit at the monitoring end and is transmitted back. To the lower computer, the lower computer controls the output of the light source to form a negative feedback control; the other light passes through the grating and irradiates the fluorescence detection area to excite and generate fluorescence, and passes through the emission monochromator and the photomultiplier tube at the detection end, and the photomultiplier tube at the detection end performs intensity detection , the electrical signal is transmitted to the lower computer through the amplifying circuit, and the lower computer transmits the detected and collected electrical signal to the upper computer, and finally a complete fluorescence spectrum is obtained in the upper computer.

Description

technical field [0001] The invention relates to the technical field of spectrum detection, in particular to a fluorescence spectrum detection system based on negative feedback regulation. Background technique [0002] Fluorescence spectroscopy has the advantages of high sensitivity, strong selectivity, and less sample usage, so it is widely used in engineering applications, such as food safety monitoring in food processing and petroleum and mineral exploration in geology. , Determination of soil mineral composition and detection of trace elements in substances, etc. [0003] At present, the common fluorescence spectrum detection system is to directly collect the fluorescence excited by the laser. However, in most assays, fluorescence detection is susceptible to interference from the external environment. It can be seen that the stability of the fluorescence spectrum detection system largely determines the correctness and high precision of the fluorescence detection results...

AI Technical Summary

Problems solved by technology

However, in most detections, fluorescence detection is susceptible to interference from the external environment

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