Method for determining biodegradation degree of crude oil

Abstract

The invention provides a method for determining the biodegradation degree of crude oil. The method comprises the following steps: 1, an ethanonoradamantane compound in a crude oil sample is subjectedto identification and quantitative analysis by means of comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometer; 2, according to identification and quantitative analysis results obtained in the step 1, the content ratios of non-substituted single-caged ethanonoradamantane and non-substituted dicaged ethanonoradamantane to n-dodecane and n-hexadecane are calculated; and 3,according to the content ratios obtained in the step 2, the biodegradation degree of the crude oil is determined. By means of the method, the biodegradation degree of the crude oil can be quantitatively determined, and the method is low in degree of dependence on personal experience and accurate in judgment on the biodegradation degree of the crude oil.

Description

technical field

[0001] The invention relates to a method for determining the degree of biodegradation of crude oil, in particular to a method for determining the degree of biodegradation of crude oil by using ethyl noradamantane compounds, and belongs to the technical field of petroleum sample analysis. Background technique

[0002] In most cases, the change in crude oil composition is mainly caused by biodegradation. According to Hunt statistics, about 1 / 5 of the crude oil in the world is destroyed by bacteria, and about 1 / 5 of the crude oil has been transformed by bacteria (see: Hunt JM. Petroleum geochemistry and geology [M]. San Franciso, 1979: 617 -618). Since Williams et al. (see: Rubinstein J A, Winters J C. Microbial alteration of crude oil in thereservoir [A]. 158th National Meeting of the American Chemical Society [C], NewYork, 1969, 86: 22-31) first recognized natural After the biodegradation of crude oil under certain conditions, the research on the biodegradat...

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