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Method for determining biodegradation degree of crude oil

A technology of biodegradation and crude oil, applied in the direction of measuring devices, instruments, and material analysis through electromagnetic means, can solve the problems of low dependence and inaccurate judgment, and achieve the effect of accurate judgment and low dependence on personal experience

Active Publication Date: 2019-11-05
PETROCHINA CO LTD
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Problems solved by technology

[0003] Because determining the degree of biodegradation of crude oil is one of the foundations of oil and gas exploration, in addition to the research on the mechanism of biodegradation of crude oil, those skilled in the art are paying more and more attention to the degree of biodegradation of crude oil. The methods of crude oil biodegradation degree basically rely on the personal experience of different professional technicians to judge. Due to the complexity and variability of crude oil biodegradation degree, the determination of crude oil biodegradation degree through empirical judgment often leads to inaccurate judgment. Therefore, currently There is an urgent need in the field to establish a method for determining the degree of biodegradation of crude oil that is less dependent on personal experience

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  • Method for determining biodegradation degree of crude oil

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Embodiment 1

[0032] This embodiment provides a method for determining the degree of biodegradation of crude oil using ethyl noradamantane compounds, wherein the method includes the following specific steps:

[0033] 1) Take 200 mg of condensate oil A with a viscosity of 1.65 mPa·s, add 40 μL of 0.5 μg / μL d16-adamantane to the condensate oil A, then add dichloromethane to 1.5 mL, mix well and set aside;

[0034] 2) Using a comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometer (GC×GC-TOF MS) to detect the mixture to identify and quantitatively analyze the ethyl noradamantane compounds in the condensate A ;

[0035] Obtain the absolute content of unsubstituted single cage ethyl noradamantane, unsubstituted double cage ethyl noradamantane, n-dodecane and n-hexadecane respectively;

[0036] 3) According to the identification and quantitative analysis results obtained in step 2), calculate the content ratio of unsubstituted single cage ethyl noradamantane and unsubst...

Embodiment 2

[0040] This embodiment provides a method for determining the degree of biodegradation of crude oil using ethyl noradamantane compounds, wherein the method includes the following specific steps:

[0041]1) Take 200 mg of condensate oil B with a viscosity of 2.65 mPa·s, add 40 μL of 0.5 μg / μL d16-adamantane to the condensate oil B, then add dichloromethane to 1.5 mL, mix well and set aside;

[0042] 2) Using a comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometer (GC×GC-TOF MS) to detect the mixture to identify and quantitatively analyze the ethyl noradamantane compounds in the condensate B ;

[0043] Obtain the absolute content of unsubstituted single cage ethyl noradamantane, unsubstituted double cage ethyl noradamantane, n-dodecane and n-hexadecane respectively;

[0044] 3) According to the identification and quantitative analysis results obtained in step 2), calculate the content ratio of unsubstituted single cage ethyl noradamantane and unsubsti...

Embodiment 3

[0048] This embodiment provides a method for determining the degree of biodegradation of crude oil using ethyl noradamantane compounds, wherein the method includes the following specific steps:

[0049] 1) Take 200 mg of crude oil C with a viscosity of 5.56 mPa·s, add 40 μL of 0.5 μg / μL d16-adamantane to the normal oil C, then add dichloromethane to 1.5 mL, mix well and set aside;

[0050] 2) Using a comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometer (GC×GC-TOF MS) to detect the mixed solution, so as to identify and quantitatively analyze the ethyl noradamantane compounds in the normal oil C;

[0051] Obtain the absolute content of unsubstituted single cage ethyl noradamantane, unsubstituted double cage ethyl noradamantane, n-dodecane and n-hexadecane respectively;

[0052] 3) According to the identification and quantitative analysis results obtained in step 2), calculate the content ratio of unsubstituted single cage ethyl noradamantane and unsu...

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Abstract

The invention provides a method for determining the biodegradation degree of crude oil. The method comprises the following steps: 1, an ethanonoradamantane compound in a crude oil sample is subjectedto identification and quantitative analysis by means of comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometer; 2, according to identification and quantitative analysis results obtained in the step 1, the content ratios of non-substituted single-caged ethanonoradamantane and non-substituted dicaged ethanonoradamantane to n-dodecane and n-hexadecane are calculated; and 3,according to the content ratios obtained in the step 2, the biodegradation degree of the crude oil is determined. By means of the method, the biodegradation degree of the crude oil can be quantitatively determined, and the method is low in degree of dependence on personal experience and accurate in judgment on the biodegradation degree of the crude oil.

Description

technical field [0001] The invention relates to a method for determining the degree of biodegradation of crude oil, in particular to a method for determining the degree of biodegradation of crude oil by using ethyl noradamantane compounds, and belongs to the technical field of petroleum sample analysis. Background technique [0002] In most cases, the change in crude oil composition is mainly caused by biodegradation. According to Hunt statistics, about 1 / 5 of the crude oil in the world is destroyed by bacteria, and about 1 / 5 of the crude oil has been transformed by bacteria (see: Hunt JM. Petroleum geochemistry and geology [M]. San Franciso, 1979: 617 -618). Since Williams et al. (see: Rubinstein J A, Winters J C. Microbial alteration of crude oil in thereservoir [A]. 158th National Meeting of the American Chemical Society [C], NewYork, 1969, 86: 22-31) first recognized natural After the biodegradation of crude oil under certain conditions, the research on the biodegradat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86G01N27/62
CPCG01N30/02G01N30/06G01N30/8679G01N27/62G01N2030/025
Inventor 王萌池林贤朱光有张志遥李婷婷陈玮岩陈志勇
Owner PETROCHINA CO LTD
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