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Cell factory pipeline system

A pipeline system and factory technology, applied in the field of cell culture, can solve the problems of reduced pressure of nutrient solution, less supply of nutrient solution in the culture tank, and reduced speed of supply of nutrient solution in the culture tank, etc., to ensure consistency and convenience. Ejection and replenishment operations, the effect of improving uniformity

Active Publication Date: 2019-11-08
SHANGHAI KING CELL BIOTECHNOLOGY CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the initial supply pressure of the nutrient solution given by the liquid storage tank to each pipeline is consistent. Due to the large volume of the culture tank, some culture tanks are often far away from the liquid storage tank, which results in some of the culture tanks that are far away from the liquid storage tank. The pressure of supplying nutrient solution becomes smaller, that is, the speed of supplying nutrient solution to some culture tanks becomes smaller, and the staff usually completes the nutrient supplement in the culture tank by timing, which in turn leads to the supply of nutrient solution to some culture tanks. Fewer, seriously affecting the consistency of cultured cells, thus seriously reducing the quality of cultured cells, there is room for improvement

Method used

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Examples

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Embodiment 2

[0050] see Figure 5 , a cell factory piping system, the difference between it and Embodiment 1 is that a supply pump 7 is provided on the lower side of the liquid storage tank 1, and the suction port of the supply pump 7 communicates with the bottom of the liquid storage tank 1, and the supply pump 7 An infusion tube 71 is connected to the outlet of the liquid outlet, and any end of the supply pipe 3 that is connected to the culture tank 2 and away from the culture tank 2 is connected in parallel to the infusion tube 71 . In actual work, the supply pump 7 draws the nutrient solution in the liquid storage tank 1 and supplies it to the culture tank 2 through the supply pipe 3 .

[0051] The tank opening of the liquid storage tank 1 is sealed with a closing cover 8, and the upper part of the closing cover 8 is connected with a vent pipe 81, and the vent pipe 81 is provided with a third electromagnetic valve 82, and the end of the vent pipe 81 away from the closing cap 8 is conne...

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Abstract

The invention discloses a cell factory pipeline system, and relates to the technical field of cell culture. The cell factory pipeline system comprises a liquid storage tank and a plurality of culturetanks, and supply tubes communicate between any of the culture tanks and the liquid storage tank. Electric flow regulating valves are disposed on the side, close to the culture tanks, of any of the supply tubes. Any of the supply tubes which are provided with feedback control flow sensors are arranged on the side, close to the culture tanks, of the electric flow regulating valves. The feedback control flow sensors are electrically connected with the corresponding electric flow regulating valves and control the electric flow regulating valves; and in actual operation, the maximum supply speed of the liquid storage tank to each supply tube is not less than the speed at which a nutrient solution actually enters the culture tanks. The cell factory pipeline system has the advantages of helpingto ensure the consistency of the supply speed and the supply amount of the nutrient solutions of the corresponding culture tanks, and helps to ensure the culture quality of cells.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a cell factory piping system. Background technique [0002] Cell culture refers to a method of simulating the in vivo environment (sterility, suitable temperature, pH and certain nutritional conditions, etc.) in vitro, so that cells can survive, grow, reproduce and maintain the main structure and function. [0003] At present, in the industrialized production of cells, in order to mass-produce the same kind of cells, several culture tanks are often required to cultivate and produce the cells at the same time. In actual operation, in order to ensure the consistency of cultured cells in several culture tanks, the nutrient solution is often uniformly configured, and the nutrient solution is placed in a general liquid storage tank; The nutrient solution is supplied in the culture tank. [0004] However, the initial supply pressure of the nutrient solution given by the liquid s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/06C12M1/36C12M1/02C12M1/00
CPCC12M23/34C12M23/58C12M29/26C12M29/04C12M41/18C12M41/44C12M41/48C12M45/02C12M37/02
Inventor 田大勇徐兵万节玲黄煜游文婷卫军军钟淑芹
Owner SHANGHAI KING CELL BIOTECHNOLOGY CO LTD
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