Bacillus amyloliquefaciens GLD-191, microbial agent of bacillus amyloliquefaciens GLD-191 and preparation method and application of bacillus amyloliquefaciens GLD-191

A technology of amylolytic spores and bacilli, which is applied in botany equipment and methods, biochemical equipment and methods, applications, etc., can solve the problems of waste disposal and waste of plant resources, and achieve improved utilization, strong antibacterial activity, and good The effect of applying the effect

Active Publication Date: 2019-11-12
陕西绿顿作物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Banlangen slag is a by-product of the extraction of antiviral agents. It is rich in organ...

Method used

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  • Bacillus amyloliquefaciens GLD-191, microbial agent of bacillus amyloliquefaciens GLD-191 and preparation method and application of bacillus amyloliquefaciens GLD-191

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Isolation of Bacillus amyloliquefaciens GLD-191

[0028] Bacillus amyloliquefaciens GLD-191 was isolated from Qinling virgin forest soil in Shaanxi Province.

[0029] The specific method is as follows: the virgin forest soil is collected in Baiyun Township, Taibai County, Shaanxi Province, air-dried naturally, and stored at room temperature for the isolation of antagonistic bacteria. 1 g of the soil was diluted with sterile water into a gradient of 10-3, 10-4, 10-5. Pipette 100 μL each into LB medium and spread evenly with a glass spreader. After culturing in an incubator at 28-30°C for 48-72 hours, bacteria with different colony shapes were picked for isolation and purification. Taking Fusarium wilt of watermelon as indicator bacteria, the antagonistic bacteria of Fusarium wilt of watermelon were screened by plate confrontation method. After 6 days of growth in the incubator at 28°C, the strains with inhibition zone were selected for further screening.

[0030] Use ...

Embodiment 2

[0032] Identification of Bacillus amyloliquefaciens GLD-191

[0033] Physiological and biochemical characteristics and 16SrDNA analysis were performed on the strain GLD-191 isolated in Example 1 according to conventional methods, and it was identified as Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) and named as Bacillus amyloliquefaciens GLD-191.

[0034] The strain GLD-191 does not produce pigment when grown on LB medium, and the colonies are flat or round, milky white and opaque, and the edges of the colonies are irregular. The surface is wrinkled. Gram-positive, rod-shaped, spore-forming, spore-elliptic or cylindrical. Refer to the "Common Bacteria Identification Manual" for physiological and biochemical identification of strains: Mannitol test positive, lactose test positive, V-P test positive, 7% NaCl growth test negative, methyl red test negative, indole test positive, pH5.7 growth test positive, citrate utilization test positive, L-arabinose test positive, ...

Embodiment 3

[0046] Control effect test of Bacillus amyloliquefaciens GLD-191 on watermelon wilt

[0047] The control effect of Bacillus amyloliquefaciens GLD-191 on potted watermelon wilt was determined with sterile water as negative control and carbendazim as common chemical control.

[0048] Put the isolated bacterial strain GLD-191 in Example 1 into a sterilized stoppered test tube containing normal saline and glass beads, then vibrate with an oscillator to form a bacterial suspension with a concentration of 1 ×10 9 CFU / ml.

[0049] Potted watermelons (a total of 90 plants, 30 plants in each group) were sprayed with sterile water, bacterial suspension, and carbendazim (diluted 1000 times according to the instructions), and inoculated with 1×10 watermelons 48 hours later. 5 cells / mL of the spore suspension of Fusarium wilt of watermelon. Moisturize in the dark for 72 hours, culture at a constant temperature of 25°C, and observe the disease. The control effect is calculated by the in...

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Abstract

The invention relates to the field of biological control and bacterial manure, in particular to bacillus amyloliquefaciens GLD-191, a microbial agent of the bacillus amyloliquefaciens GLD-191 and a preparation method and application of the bacillus amyloliquefaciens GLD-191. The bacillus amyloliquefaciens GLD-191 strain is deposited in the China General Microbiological Culture Collection Center onDecember 19, 2018, and the preservation number is CGMCC No.17011. The invention further provides the microbial agent prepared from the bacillus amyloliquefaciens GLD-191, a preparation method of themicrobial agent, and application of the bacillus amyloliquefaciens GLD-191 and the microbial agent thereof in the prevention and treatment of plant diseases. The bacillus amyloliquefaciens GLD-191 andan isatis root slag solid medium are subjected to secondary fermentation to prepare the novel microbial agent, the utilization rate of nutrients in isatis root slag is improved, and the bacillus amylolyticus GLD-191 can produce antibacterial substances with antimicrobial activity against plant pathogenic fungi, the population of beneficial microorganisms is increased, and the spectrum of diseasecontrol is expanded.

Description

technical field [0001] The invention relates to the field of biological control and microbial fertilizer, in particular to Bacillus amyloliquefaciens GLD-191 and its bacterial agent, preparation method and application. Background technique [0002] Bacillus amyloliquefaciens itself has the ability to inhibit fungi and bacteria extensively, and can change the environment and types of the surrounding flora, so it has become the most potential microbial species for biocides and microbial fertilizers. A large number of international studies have found that Bacillus amyloliquefaciens can produce antibacterial substances that have antibacterial activity against plant pathogenic fungi. These antibacterial substances mainly include low molecular weight antibiotics such as peptides, lipopeptides, bacteriocins, and high molecular weight protein antibacterial substances. . [0003] Radix isatidis slag is a by-product of the extraction of antiviral agents, which is rich in organic matt...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N25/08A01N63/02A01P3/00A01P21/00C09K17/14C12R1/07
CPCC12N1/20A01N25/08C09K17/14C12R2001/07C12N1/205Y02W30/40
Inventor 同中平安航同高鹏同小蕊安德荣
Owner 陕西绿顿作物科技有限公司
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