Bacillus amyloliquefaciens GLD-191, microbial agent of bacillus amyloliquefaciens GLD-191 and preparation method and application of bacillus amyloliquefaciens GLD-191
A technology of amylolytic spores and bacilli, which is applied in botany equipment and methods, biochemical equipment and methods, applications, etc., can solve the problems of waste disposal and waste of plant resources, and achieve improved utilization, strong antibacterial activity, and good The effect of applying the effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0027] Isolation of Bacillus amyloliquefaciens GLD-191
[0028] Bacillus amyloliquefaciens GLD-191 was isolated from Qinling virgin forest soil in Shaanxi Province.
[0029] The specific method is as follows: the virgin forest soil is collected in Baiyun Township, Taibai County, Shaanxi Province, air-dried naturally, and stored at room temperature for the isolation of antagonistic bacteria. 1 g of the soil was diluted with sterile water into a gradient of 10-3, 10-4, 10-5. Pipette 100 μL each into LB medium and spread evenly with a glass spreader. After culturing in an incubator at 28-30°C for 48-72 hours, bacteria with different colony shapes were picked for isolation and purification. Taking Fusarium wilt of watermelon as indicator bacteria, the antagonistic bacteria of Fusarium wilt of watermelon were screened by plate confrontation method. After 6 days of growth in the incubator at 28°C, the strains with inhibition zone were selected for further screening.
[0030] Use ...
Embodiment 2
[0032] Identification of Bacillus amyloliquefaciens GLD-191
[0033] Physiological and biochemical characteristics and 16SrDNA analysis were performed on the strain GLD-191 isolated in Example 1 according to conventional methods, and it was identified as Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) and named as Bacillus amyloliquefaciens GLD-191.
[0034] The strain GLD-191 does not produce pigment when grown on LB medium, and the colonies are flat or round, milky white and opaque, and the edges of the colonies are irregular. The surface is wrinkled. Gram-positive, rod-shaped, spore-forming, spore-elliptic or cylindrical. Refer to the "Common Bacteria Identification Manual" for physiological and biochemical identification of strains: Mannitol test positive, lactose test positive, V-P test positive, 7% NaCl growth test negative, methyl red test negative, indole test positive, pH5.7 growth test positive, citrate utilization test positive, L-arabinose test positive, ...
Embodiment 3
[0046] Control effect test of Bacillus amyloliquefaciens GLD-191 on watermelon wilt
[0047] The control effect of Bacillus amyloliquefaciens GLD-191 on potted watermelon wilt was determined with sterile water as negative control and carbendazim as common chemical control.
[0048] Put the isolated bacterial strain GLD-191 in Example 1 into a sterilized stoppered test tube containing normal saline and glass beads, then vibrate with an oscillator to form a bacterial suspension with a concentration of 1 ×10 9 CFU / ml.
[0049] Potted watermelons (a total of 90 plants, 30 plants in each group) were sprayed with sterile water, bacterial suspension, and carbendazim (diluted 1000 times according to the instructions), and inoculated with 1×10 watermelons 48 hours later. 5 cells / mL of the spore suspension of Fusarium wilt of watermelon. Moisturize in the dark for 72 hours, culture at a constant temperature of 25°C, and observe the disease. The control effect is calculated by the in...
PUM
Property | Measurement | Unit |
---|---|---|
Diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com