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Fugu rubripe triploid induction method based on hydrostatic pressure

A technology of redfin puffer and triploid, applied in fish farming, application, climate change adaptation, etc., can solve unrealistic, labor-intensive and time-consuming, uneconomical problems, and achieve simple operation, high success rate, time-saving effect

Pending Publication Date: 2019-11-15
大连天正实业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the mature season, experienced people can distinguish between male and female by touching the belly of the fish, but it is not 100% accurate
When selling tens of thousands of quantities, distinguishing males and females by touching the belly of each tail requires huge labor and time, which is not economical
In addition, the method of molecular markers can accurately identify males and females, but tens of thousands of numbers can be identified by DNA methods, which is also unrealistic in terms of time and cost.

Method used

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  • Fugu rubripe triploid induction method based on hydrostatic pressure
  • Fugu rubripe triploid induction method based on hydrostatic pressure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for inducing triploids of the red-finned puffer based on a hydrostatic pressure method, the method for inducing the triploids of the red-finned puffer comprises the following steps:

[0021] Using the method of artificial induction to obtain the semen of the mature male fish of the red-finned puffer and the mature eggs of the female fish of the red-finned puffer;

[0022] Add semen to the mature eggs, stir to make the sperm fully contact with the eggs, then add sea water to activate the eggs, and obtain fertilized eggs. The temperature of the fertilized water is 17°C, the pH is 8.0, and the salinity is 30‰;

[0023] 4 minutes after adding seawater, transfer the fertilized eggs to the pressurized chamber of the hydrostatic press, and apply a pressure of 40MPa for 2 minutes;

[0024] Transfer the fertilized eggs after hydrostatic pressure treatment to an incubator for incubation. The temperature of the incubation water is 17°C, the pH is 8.0, and the salinity is ...

Embodiment 2

[0027] A method for inducing triploids of the red-finned puffer based on a hydrostatic pressure method, the method for inducing the triploids of the red-finned puffer comprises the following steps:

[0028] Using the method of artificial induction to obtain the semen of the mature male fish of the red-finned puffer and the mature eggs of the female fish of the red-finned puffer;

[0029] Add semen to the mature eggs, stir to make the sperm fully contact with the eggs, then add sea water to activate the eggs, and obtain fertilized eggs. The temperature of the fertilized water is 18°C, the pH is 7.8, and the salinity is 28‰;

[0030] 4 minutes after adding seawater, transfer the fertilized eggs to the pressurized chamber of the hydrostatic press, and apply a pressure of 50MPa for 5 minutes;

[0031] Transfer the fertilized eggs after hydrostatic pressure treatment to the incubator for incubation, the incubation water temperature is 18°C, the pH is 7.8, and the salinity is 28‰. ...

Embodiment 3

[0034] A method for inducing triploids of the red-finned puffer based on a hydrostatic pressure method, the method for inducing the triploids of the red-finned puffer comprises the following steps:

[0035] Using the method of artificial induction to obtain the semen of the mature male fish of the red-finned puffer and the mature eggs of the female fish of the red-finned puffer;

[0036] Add semen to the mature eggs, stir to make the sperm fully contact with the eggs, then add sea water to activate the eggs, and obtain fertilized eggs. The temperature of the fertilized water is 20°C, the pH is 8.1, and the salinity is 31‰;

[0037] 6 minutes after adding seawater, transfer the fertilized eggs to the pressurized chamber of the hydrostatic press, and apply a pressure of 40MPa for 2 minutes;

[0038] Transfer the fertilized eggs after hydrostatic pressure treatment to an incubator for incubation. The temperature of the incubation water is 20°C, the pH is 8.1, and the salinity is ...

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Abstract

The invention relates to a fugu rubripe triploid induction method based on hydrostatic pressure, and belongs to the field of genetic breeding of fugu rubripes. The fugu rubripe triploid induction method based on hydrostatic pressure includes the following steps that sperms of mature male fugu rubripes and mature ova of female fugu rubripes are taken respectively; the sperms are added into the mature ova, and sea water is added to obtain fertilized ova; the fertilized ova are transferred to a pressurization chamber of a hydrostatic press, and the pressure of 30-80 MPa is applied for 2-10 min; the fertilized ova are transferred to an incubator for incubation after hydrostatic pressure treatment. The method has high success rate of triploid induction, high incubation rate and simple operation, time is saved, the practicability and the operability are good, and the method can be used as an industrial application measure.

Description

technical field [0001] The invention relates to a triploid induction method for red-finned puffers based on a hydrostatic pressure method, and belongs to the field of genetics and breeding of red-finned puffers. Background technique [0002] The red-finned puffer (Takifugu rubripes) belongs to the Fuguformes, Pufferidae, and the genus Takifugu. It is distributed in Japan, the Korean Peninsula and the coast of China in the western North Pacific Ocean. It is found in the Yellow Sea and the East China Sea in China. At present, 17 species of puffer have been found along the coast of my country, and the red-finned puffer is the largest individual in this genus and has a high economic value. Because of its tender meat, delicious taste, high protein and rich nutrition, it is deeply loved by gourmets, especially famous for its delicious sashimi. The muscle of pufferfish is edible, but its gonads, viscera, skin, etc. are poisonous. The ovaries of mature females account for about 20...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/17
CPCA01K61/17Y02A40/81
Inventor 刘海金刘圣聪包玉龙孙群汶杨淑林孙立刘忠强张涛杨君王宁孟雪松
Owner 大连天正实业有限公司
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