Micro-fluidic chip and preparation method and capture method thereof

Abstract

The invention discloses a micro-fluidic chip and a preparation method and capture method thereof. The micro-fluidic chip comprises a micro-fluidic channel film (1), a substrate (4) and a response hydrogel micro-structure array (5), wherein a micro-fluidic channel (2) in a preset shape is arranged in the micro-fluidic channel film (1), each end point of the micro-fluidic channel (2) is provided with a through hole (3) running through the micro-fluidic channel (2), the substrate (4) is bonded to the micro-fluidic channel film (1) so as to close the micro-fluidic channel film (1), the response hydrogel micro-structure array (5) is a closed structure composed of a response hydrogel microstructure (6) and is arranged in the micro-fluidic channel (2), and the size of the response hydrogel microstructure (6) is controlled by the located environment. By adopting the response hydrogel microstructure with the adjustable size, objects are captured by controlling the intervals of the response hydrogel microstructure array, and no external device needs to be connected.

Description

technical field [0001] The invention relates to the field of chip devices, in particular to a microfluidic chip, a preparation method and a capture method thereof. Background technique [0002] There are differences in gene transcription, translation, protein activity and metabolic abundance among different individual cells. When single cell genomes such as single nucleotide variation (SNVs), copy number variation (CNVs) and structural variation, it is likely to lead to cancer and other diseases. In the traditional cell research, the characteristic information is obtained by the cell population, and the difference between individual cells cannot be reflected. Therefore, it is of great significance to separate and extract single cells from cell populations, realize the capture of single cells, and then study and analyze single cells in the fields of neuroscience, stem cell biology, and cancer diagnosis. [0003] Cell separation technology based on microfluidic chip can be d...

AI Technical Summary

Problems solved by technology

Active technology relies on external positions such as light, magnetism, sound and electricity to achieve cell separation. Therefore, when active technology realizes cell separation, it is necessary to connect devices that provide external force fields on the microfluidic chip, and these devices are often large in size and structurally difficult. Complicated and expensive, contrary to the concept of microfluidic chips

Passive technology relies on the geometry of microfluidic channels and inherent fluid dynamics to achieve cell separation without connecting external devices, but this passive technology is unstable. Once the fluid changes, the captured cells can easily get out of the trap, which is extremely difficult. Large limitations limit its application in single-cell research

Images