Cervus nippon heart protein hydrolysate and preparation method and application thereof

Abstract

The invention relates to cervus nippon heart protein hydrolysate and a preparation method and application thereof, and belongs to the technical field of biology medicines. The preparation method comprises the following steps of performing pretreatment: taking cervus nippon hearts, adding normal saline, and performing tissue homogenization to obtain an enzymolysis stock solution for standby application; performing enzymolysis: taking the enzymolysis stock solution, adding pepsin, and performing an enzymolysis reaction so as to obtain enzymatic hydrolysate; and performing purification: taking the enzymatic hydrolysate, performing separation and purification with a polydextran gel column, and taking polypeptide of 3KD-15KD so as to obtain the cervus nippon heart protein hydrolysate. Accordingto the cervus nippon heart protein hydrolysate disclosed by the invention, cervus nippon hearts are used as raw materials, through tissue homogenization treatment, an appropriate enzymolysis method is adopted, and micromolecule polypeptide substances are obtained. An aged cardiac muscle cell model is established, influence of various enzymolysis products on the proliferation rate of cardiac muscle cells is detected by an MTT method, and the experiment confirms that the obtained cervus nippon heart protein hydrolysate has better activity on relieving ageing of the cardiac muscle cells.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a deer heart protein hydrolyzate and its preparation method and application. Background technique [0002] Sika deer (Cervus nippon) is a rare medicinal animal that is special in the monsoon region of East Asia. It is used for meat, skin and ornamental, and has high economic value. Deer heart is a good product for invigorating qi and calming the nerves. According to the traditional Chinese medicine theory of nourishing shape, deer heart can treat rheumatic heart disease, heart failure, palpitation and palpitation and other diseases related to the heart. With its unique nutritional value and traditional The habit of eating deer heart has always been favored by experts and scholars, and it has become a hot spot in the study of heart disease and anti-aging. [0003] However, the sika deer heart is used in the folk compatibility of Chinese herbal medicines, in the form of pills ...

AI Technical Summary

Problems solved by technology

The traditional deer heart is often used as a precious medicinal material by humans to cook into delicacies. At present, there is no in-depth research on sika deer in modern medicine.

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