Divalent targeted polypeptide probe and preparation method thereof

A technology for targeting peptides and probes, applied in the field of bivalent targeting peptide probes and their preparation, can solve the problems of weak affinity between peptides and target molecules, etc.
CN110456053AActive Publication Date: 2019-11-15INST OF CHEM CHINESE ACAD OF SCI +1
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Patent Information

Authority / Receiving Office
CN ยท China
Current Assignee / Owner
INST OF CHEM CHINESE ACAD OF SCI
Publication Date
2019-11-15

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Abstract

The invention discloses a divalent targeted polypeptide probe and a preparation method and application thereof. The probe comprises A) two tumor specific targeting polypeptide; B) two PEG linking arms; C) skeleton core peptide and D) fluorescence molecules. Each tumor specific targeting polypeptide is connected with the PEG linking arm via a group as shown in the formula I; the amino acid sequenceof the skeleton core peptide is KGGKG; the skeleton core peptide is coupled to the two tumor specific targeting polypeptide via the PEG linking arms; and the fluorescence molecules are connected to the skeleton core peptide via chemical bonds. According to the multivalent targeted probe FITC-2PEG9-2AP2H, two targeted ligands AP2H are coupled to the skeleton core peptide via PEG chains, the PEG chains can improve the dissolvability of the targeted polypeptide probe in an aqueous solution as well as the flexibility of the probe, and thus, the probe can identify and be combined with a target object.
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Description

technical field

[0001] The invention relates to a bivalent targeting polypeptide probe and a preparation method thereof. Background technique

[0002] Malignant tumors are a serious threat to human life and health. Because their pathogenesis has not been fully elucidated, there have always been problems such as difficulty in early diagnosis, few innovative drugs at the source, and lack of effective treatment methods. Early diagnosis and treatment of malignant tumors can significantly improve patient survival and cure rate. At present, the diagnosis and treatment methods based on the specific recognition principle of antibodies and tumor markers are very effective methods in tumor diagnosis and treatment. Antibodies have the advantages of high affinity and strong specificity. Immunogenicity. Therefore, in recent years, more and more small-molecule targeting ligands, such as peptide ligands and nucleic acid aptamers, have attracted extensive attention from researchers, have ...

Claims

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