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A kind of method of Polygonatum multiflora rooting and promoting budding

A technology of Rhizoma Polygonatum and bottle seedlings, which is applied in the field of plant tissue culture, can solve problems such as poor results, and achieve the effects of reducing interference from external factors, high safety, and fast growth

Active Publication Date: 2021-04-06
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The inventor of the present application once sprayed plant growth regulator on the rhizome of Polygonatum polyflora and carried out experiments, the purpose is to promote the germination of side buds on the rhizome, but the effect is not good

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A method for promoting rooting and sprouting of Polygonatum multiflora, comprising the following steps:

[0017] (1) Use of bud inducer: On the ultra-clean workbench, sterilize the surface of the bottle of Polygonatum multiflora rooting seedlings with 75% ethanol, open the bottle cap, and spray the seedlings with the sterilized bud inducer At the same time, add 2 ml of bud inducer on the surface of the culture medium, shake the bottle gently, let the bud inducer evenly spread on the culture medium, and cover the bottle cap;

[0018] The bud-inducing agent is composed of 0.05 mg / L thiadiazide urea, 0.1 mg / L zeatin, and 0.1 mg / L naphthaleneacetic acid;

[0019] (2) Bud induction culture: Put the rooted bottle seedlings of Polygonatum multiflora treated in step (1) in the culture room for 7 days, move them out to the outside, and continue to cultivate them on the shelf of the greenhouse; Begin to grow lateral buds on the rhizomes of root seedlings, and when cultivating to...

Embodiment 2

[0022] A method for promoting rooting and sprouting of Polygonatum multiflora, comprising the following steps:

[0023] (1) Use of bud inducer: On the ultra-clean workbench, sterilize the surface of the bottle of Polygonatum multiflora rooting seedlings with 75% ethanol, open the bottle cap, and spray the seedlings with the sterilized bud inducer At the same time, add 2 ml of bud inducer on the surface of the culture medium, shake the bottle gently, let the bud inducer evenly spread on the culture medium, and cover the bottle cap;

[0024] Described bud inducer is made up of 0.2 mg / L thiadiazide urea, 0.5 mg / L zeatin, 0.5 mg / L naphthaleneacetic acid;

[0025] (2) Cultivation of bud induction: Put the rooted bottle seedlings of Polygonatum multiflora treated in step (1) in the cultivation room for 7 days, move them out to the outside, and continue to cultivate them on the shelf of the greenhouse; On the rhizome of rooted seedlings, lateral buds begin to grow, and when being cu...

Embodiment 3

[0028] A method for promoting rooting and sprouting of Polygonatum multiflora, comprising the following steps:

[0029] (1) Use of bud inducer: On the ultra-clean workbench, sterilize the surface of the bottle of Polygonatum multiflora rooting seedlings with 75% ethanol, open the bottle cap, and spray the seedlings with the sterilized bud inducer At the same time, add 2 ml of bud inducer on the surface of the culture medium, shake the bottle gently, let the bud inducer evenly spread on the culture medium, and cover the bottle cap;

[0030] Described bud inducer is made up of 0.1 mg / L thiadiazide urea, 0.3 mg / L zeatin, 0.3 mg / L naphthaleneacetic acid;

[0031] (2) Cultivation of bud induction: Put the rooted bottle seedlings of Polygonatum multiflora treated in step (1) in the cultivation room for 7 days, move them out to the outside, and continue to cultivate them on the shelf of the greenhouse; Begin to grow lateral buds on the rhizome of rooted seedlings; when cultivating t...

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PUM

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Abstract

The invention discloses a method for promoting sprouting of Polygonatum multiflora rooting seedlings. After rooting of Polygonatum multiflora tissue-cultured seedlings is completed, the rooting seedlings are left in a culture bottle, and the leaves and leaves of the rooting seedlings are sprayed with a sterilized bud inducer. The buds are also added to the culture medium; the rooted shoots are left in the bottle to continue culturing, so as to obtain multi-bud Polygonatum tissue culture seedlings. The present invention carries out multi-bud induction in the Polygonatum polyflora tissue culture link, reduces the interference of external factors, and provides controllability for the experiment; although the time is increased by one month, the output of the tissue culture seedlings is that of the general tissue culture seedlings N times the output, and the month of budding cultivation is cultivated in the greenhouse, which does not occupy the space of the cultivation room; the operation is simple and easy, efficient and stable, reducing investment, increasing production, reducing costs, saving manpower and material resources and other resources.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a method for promoting rooting and budding of Polygonatum multiflora. Background technique [0002] Polygonatum (Polygonatum cyrtonema Flower) is a perennial herb of Liliaceae Polygonatum. It is a large number of Chinese medicinal materials in my country, and it is also a medicinal and edible plant resource. Polygonatum multiflora is distributed in Zhejiang, Jiangsu, Hunan, Anhui, Guangxi, Jiangxi and other places, with a wide range of distribution and large wild reserves. At present, medicinal resources are still dominated by wild resources. Due to the serious phenomenon of random mining and indiscriminate digging, the output of wild resources is declining year by year. [0003] The rhizome of Polygonatum polyflora is the medicinal part, and it is also the composition of the output of Polygonatum polyflora. One rhizome only grows one main bud every year, and there are few ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01G7/06A01G31/00
Inventor 石云平林茜桂杰许娟苏祖祥韦绍龙李小泉
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI