Oxygen-producing hydrogel and preparation method and application thereof

A hydrogel and gel technology, applied in the field of oxygen-producing hydrogel and its preparation, can solve the problems of reduced anti-tumor effect of photodynamic therapy, uneven distribution of oxygen concentration, and large oxygen consumption, so as to avoid low targeting Problems, improvement of hypoxic environment, effect of increasing oxygen production

Inactive Publication Date: 2019-11-19
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Currently, most of the existing photodynamic therapy systems are highly oxygen-dependent and need to consume a large amount of oxygen
However, due to the excessive proliferation of tumor cells and insufficient blood supply inside solid tumors, the distribution of oxygen concentration in solid tumors is uneven, and the oxygen concentration in some internal areas is very low, and the oxygen partial pressure is even less than 5mmHg
Insufficient oxygen in the tumor leads to a significant reduction in the antitumor effect of photodynamic therapy, especially when multiple photodynamic therapy sessions are required

Method used

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  • Oxygen-producing hydrogel and preparation method and application thereof
  • Oxygen-producing hydrogel and preparation method and application thereof
  • Oxygen-producing hydrogel and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0053] The preparation scheme of oxygen-producing hydrogel is as follows:

[0054] Prepare photosynthetic microalgae medium, autoclave at 121°C for 30 minutes, insert Chlorella pyrenoidosa species, and culture statically for 7 days. Give 12 hours of light and 12 hours of dark treatment every day. The chlorella pyrenoidosa was processed in the ultra-clean bench, and the chlorella pyrenoidosa was collected by centrifugation at a speed of 2500 rpm. Dilute the concentrated algae liquid, measure its UV absorbance at 680nm, and calculate the concentration of microalgae. Prepare 5% sodium alginate solution, after autoclaving at 121°C for 30 minutes, mix the sodium alginate solution and the concentrated solution of Chlorella pyrenoidosa in a volume ratio of 1:1 to obtain sodium alginate and pyrenoid pellets Algae mixed solution, weigh calcium chloride and use normal saline to prepare 1% (w / v) calcium chloride normal saline solution. Use a 1 ml syringe to drop the mixed solution of ...

Embodiment 2

[0062] Oxygen production of oxygen-producing hydrogels under light. Using phosphate buffer as the measurement medium, the dissolved oxygen meter was used to measure the oxygen content in the phosphate buffer under the conditions of adding oxygen-producing hydrogel and not adding oxygen-producing hydrogel, giving light and dark treatment conditions under agitation. The light is 635nm visible light, and the light dose is 50mW cm -2 . figure 2 Oxygen production of oxygen-producing hydrogels under light and dark conditions.

Embodiment 3

[0064] Determination of repeated oxygen production capacity of oxygen-producing hydrogels. Stabilize the readings of the dissolved oxygen meter under dark conditions, repeat the experimental steps of adding the oxygen-generating gel to give light in Example 2, repeat four times, and measure the oxygen production of the oxygen-generating gel under the same light conditions. image 3 Oxygen production for oxygen-producing hydrogels subjected to multiple light exposures.

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Abstract

The invention discloses an oxygen-producing hydrogel and a preparation method and application thereof. The oxygen-producing hydrogel comprises photosynthetic microalgae and biodegradable hydrogel, andby wrapping the photosynthetic microalgae with the hydrogel good in biocompatibility, the oxygen-producing hydrogel is formed; the photosynthetic microalgae in the hydrogel performs oxygen producingby means of photolysis of water, the local oxygen yield is increased so as to improve the anoxic microenvironment of the location, and then the curative effect of treatment of hypoxic diseases is enhanced. Due to protection of the gel, the photosynthetic microalgae can keep the oxygen activity in the body for a long time, and the requirements of long-time repeated oxygen supply for the hypoxic diseases are met.

Description

technical field [0001] The invention relates to an oxygen-producing hydrogel and a preparation method and application thereof, belonging to the technical field of pharmaceutical preparations. Background technique [0002] The incidence and mortality of cancer in my country have been rising, making cancer the main cause of death since 2010, and also a major public health problem in my country, posing a huge threat to the maintenance of human life and health. To meet this challenge, research and development of a variety of treatment methods, such as surgery, chemotherapy, radiation therapy, gene therapy, biological therapy, phototherapy and immunotherapy and so on. Among the existing treatment methods, photodynamic therapy (PDT) has gradually become a promising treatment method due to the characteristics of low toxicity, high targeting and no drug resistance, and is favored by researchers and clinicians. of great concern. Photodynamic therapy uses specific wavelengths of lig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K36/05A61P35/00A61P17/02A61P29/00A61P19/02C08J3/075C08L5/04
CPCA61K9/06A61K36/05A61P17/02A61P19/02A61P29/00A61P35/00C08J3/075C08J2305/04
Inventor 姜虎林周天娇邢磊李玲
Owner CHINA PHARM UNIV
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