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Application of mdcol Gene of Cylindrical Apple in Improving Stress Resistance of Plants

An apple and column-shaped technology, applied in the biological field, can solve the problems that no research has shown that MdCoL and stress resistance, MdCoL is not deep, etc.

Active Publication Date: 2022-05-31
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the functional research on MdCoL is not in-depth, and no studies have shown that MdCoL is related to stress resistance, especially salt tolerance

Method used

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  • Application of mdcol Gene of Cylindrical Apple in Improving Stress Resistance of Plants
  • Application of mdcol Gene of Cylindrical Apple in Improving Stress Resistance of Plants
  • Application of mdcol Gene of Cylindrical Apple in Improving Stress Resistance of Plants

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[0059] MdCoL-R: GCTACTCGAGAGGCTTAAAA (SEQ ID No. 4).

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Abstract

This application discloses the application of columnar apple MdCoL gene in improving plant stress resistance. The study of the present invention proves that MdCoL transgenic tobacco seedlings and transgenic apple callus exhibit stronger salt stress tolerance and lower sensitivity to ABA, specifically as compared with WT: increased chlorophyll content and ROS accumulation decreased, SOD activity increased, MDA content decreased, and transcriptional levels of stress-related genes such as MdP5CS, MdRD22, MdDREB2, and MdNCED3 increased. The invention provides a new way for the cultivation of stress-resistant plants, is of great significance for exploring the function of MdCoL and revealing the formation of columnar traits of columnar apples, and provides an important reference for molecular breeding of columnar apples.

Description

Application of Columnar Apple MdCoL Gene in Improving Plant Stress Resistance technical field The present invention relates to the field of biotechnology, specifically a kind of columnar apple MdCoL protein and its encoding gene in the Application in high plant salt tolerance and ABA stress. Background technique Columnar (Columnar) apple (Malus×domestica Borkh) grows compactly, internode is short, and its axillary bud sprouts Hair as numerous fruiting short shoots. With little or no lateral growth to extend shoots, the tree type exhibits a solitary upright trunk. This tree It is especially suitable for high-density planting and can reduce manual labor, basically does not require fruit tree pruning, and is more conducive to mechanized management. In addition, columnar apples also have the characteristics of larger leaf area, thicker leaves and higher chlorophyll content, which can not only enhance the light exposure Energy absorption and utilization, and can improv...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00A01H6/74A01H6/82
CPCC07K14/415C12N15/8273C12N15/8293
Inventor 张玉刚孙欣文翠萍孙晓红柏素花侯鸿敏祝军戴洪义
Owner QINGDAO AGRI UNIV
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