Preparation method of mouse with specific Raptor knocked out in beta cells during GFP tracing
A specific, β-cell technology, applied in animal husbandry and other fields, can solve problems such as unclear mTORC1β cell identity attributes, glucose intolerance, etc.
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[0023] 1. βRap KO GFP mouse build
[0024] SPF (Specific pathogen Free, no specific pathogen) grade Raptor lox / lox C57BL / 6 mice and ROSA26-EGFP f Mixed background mice were purchased from Jackson Laboratory.
[0025]The 8-week-old sexually mature male mice were housed with the female mice, and separated into cages after the 3-week-old mice were born. At the same time, a small amount of nail tissue was cut and numbered for subsequent genotype identification tests. Mate ROSA26-EGFPf with RIP-Cre mice to obtain homozygous mice with RIP-Cre and EGFP sequences. The efficiency of mouse GFP-positive cell sorting was verified by flow sorting technology. Homozygous mice with RIP-Cre and EGFP sequences and Raptor in the same way lox / lox Raptor with homozygous sequences of RIP-Cre and EGFP obtained after mating lox / w mice. The Raptor heterozygous mice were then selfed to obtain Raptor with RIP-Cre and EGFP homozygous sequences lox / lox and Raptor w / w mice, the former is the mouse...
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