Compound microbial agent preventing continuous cropping disease

A kind of technology of compound microbial inoculum and microbial bacteria

Inactive Publication Date: 2019-11-22
郑州海绿王绿色科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in farmlands suffering from heavy stubble disease, in addition to the imbalance of soil nutrition and the accumulation of pathogenic bacteria of this crop caused by single planting of one crop for many years, there are also toxins produced by the decomposition of crop residues, such as organic acids, aldehydes, alcohols, Hydrocarbons, etc. cause self-toxicity, affecting the growth of crops themselves

Method used

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  • Compound microbial agent preventing continuous cropping disease
  • Compound microbial agent preventing continuous cropping disease
  • Compound microbial agent preventing continuous cropping disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The preparation method of the present invention's anti-cropping composite microbial bacterial agent comprises the following steps:

[0037] (1) Preparation of microbial inoculum:

[0038] 4mL of Bacillus licheniformis, 3mL of Klebsiella indica, 2mL of Bacillus thuringiensis, 2mL of Paecilomyces lilacinus, and 1mL of Bacillus jelly-like were cultured to obtain fermented seeds. Add 5kg of agar powder, 12kg of mannitol, 2kg of magnesium sulfate, 2kg of molasses, 4kg of beef extract, 3kg of sodium chloride, 0.15kg of potassium dihydrogen phosphate, and 0.6kg of ammonium molybdate in a 1000L fermenter, and the balance is water; Sterilize and culture at pH 7.3 in a fermenter at 115°C for 20 minutes. When the temperature of the medium drops to about 30°C, add Bacillus licheniformis, Klebsiella indica, Bacillus thuringiensis, Paecilomyces lilacinus, gum The fermented seeds of Bacillus frozen-like were inoculated into sterilized fermenters respectively, and fermented for 30 hou...

Embodiment 2

[0043] The preparation method of the present invention's anti-cropping composite microbial bacterial agent comprises the following steps:

[0044] (1) Preparation of microbial inoculum:

[0045] 3mL of Bacillus licheniformis, 2mL of Klebsiella indica, 3mL of Bacillus thuringiensis, 1mL of Paecilomyces lilacinus, and 2mL of Bacillus jelly-like were cultured to obtain fermented seeds. Add 6kg of agar powder, 10kg of mannitol, 2kg of magnesium sulfate, 2kg of molasses, 3kg of beef extract, 2kg of sodium chloride, 0.15kg of potassium dihydrogen phosphate, and 0.6kg of ammonium molybdate in a 1000L fermenter, and the balance is water; Sterilize and culture at pH 7.3 in a fermenter at 115°C for 20 minutes. When the temperature of the medium drops to about 30°C, add Bacillus licheniformis, Klebsiella indica, Bacillus thuringiensis, Paecilomyces lilacinus, gum The fermented seeds of Bacillus frozen sample were inoculated into sterilized fermenters respectively, and fermented for 36 h...

Embodiment 3

[0050] The preparation method of the present invention's anti-cropping composite microbial bacterial agent comprises the following steps:

[0051] (1) Preparation of microbial inoculum:

[0052] 4 mL of Bacillus licheniformis, 3 mL of Bacillus indica, 3 mL of Bacillus thuringiensis, 1 mL of Paecilomyces lilacinus, and 1 mL of Bacillus jelly-like were cultured to obtain fermented seeds. Add 5kg of agar powder, 14kg of mannitol, 1kg of magnesium sulfate, 2kg of molasses, 4kg of beef extract, 1kg of sodium chloride, 0.15kg of potassium dihydrogen phosphate, and 0.6kg of ammonium molybdate in a 1000L fermenter, and the balance is water; pH 7.3 Sterilize and cultivate in a fermenter at 115°C for 20 minutes. When the temperature of the medium drops to about 30°C, add Bacillus licheniformis, Klebsiella indica, Bacillus thuringiensis, Paecilomyces lilacinus, gum The fermented seeds of Bacillus frozen sample were inoculated into sterilized fermenters respectively, and fermented for 36...

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Abstract

The invention provides a compound microbial agent preventing continuous cropping disease. The compound microbial agent is prepared from the following raw materials in parts by weight: 40-50 parts of amicrobe composition, 20-25 parts of tobacco residues, 10-15 parts of humic acid, 5-10 parts of plant ash, 3-6 parts of a boron-magnesium fertilizer and 1-3 parts of trace elements. The microbe composition is prepared by mixing Bacillus licheniformis, Beijerinckia indica, Bacillus thuringiensis, Paecilomyces lilacinus and Bacillus mucilaginosus according to a mass ratio of (3-4):(2-3):(2-3):(1-2):(1-2). The compound microbial agent is prepared by uniformly mixing the microbe composition, the tobacco residues, the humic acid, the plant ash, the boron-magnesium fertilizer and the trace elementsaccording to the ratio, adding water, uniformly mixing the mixture until the mixture contains water and water in the mixture does not drop when the mixture is tightly held in a hand, putting the mixedmicrobial agent into a greenhouse for fermentation at 37 DEG C for 3-5 days, turning over the mixture for 1-3 times every day, and stacking the mixture for 2-4 days for fermentation until the temperature is 40-45 DEG C to obtain a final product. The compound microbial agent has a good synergistic effect and an excellent continuous cropping disease prevention effect, and especially can solve the problems of frequent occurrence of disease and pests and pathogenic bacteria, reduction of quality and yields, lack of organic matters and trace elements and the like in the growth of crops.

Description

technical field [0001] The invention relates to a method for regulating soil structure through a compound microbial agent to prevent heavy cropping, and specifically refers to a method suitable for regulating crop heavy cropping plots, and using a compound microbial agent to adjust soil structure to prevent heavy cropping. Background technique [0002] Heavy cropping disease is a complex problem that plagues sustainable agricultural production. It has been reported that the aggravation of soil fungal diseases, autotoxicity, increase in nematodes, one-sided absorption of soil nutrients by crops, and deterioration of soil physical and chemical properties are the main reasons for heavy cropping disease. However, the root cause is the deterioration of the soil micro-ecological environment. Continuous cropping changes the microbial ecological environment in the rhizosphere of crops to a direction that is conducive to the increase of the number of pathogenic species and the reducti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/04C05G3/02C05G3/00C12N1/20C12N1/14C12R1/01C12R1/07C12R1/79
CPCC05C3/00C05G3/00C12N1/14C12N1/20
Inventor 刘广照
Owner 郑州海绿王绿色科技有限公司
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