Compound microbial agent preventing continuous cropping disease
A kind of technology of compound microbial inoculum and microbial bacteria
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Embodiment 1
[0036] The preparation method of the present invention's anti-cropping composite microbial bacterial agent comprises the following steps:
[0037] (1) Preparation of microbial inoculum:
[0038] 4mL of Bacillus licheniformis, 3mL of Klebsiella indica, 2mL of Bacillus thuringiensis, 2mL of Paecilomyces lilacinus, and 1mL of Bacillus jelly-like were cultured to obtain fermented seeds. Add 5kg of agar powder, 12kg of mannitol, 2kg of magnesium sulfate, 2kg of molasses, 4kg of beef extract, 3kg of sodium chloride, 0.15kg of potassium dihydrogen phosphate, and 0.6kg of ammonium molybdate in a 1000L fermenter, and the balance is water; Sterilize and culture at pH 7.3 in a fermenter at 115°C for 20 minutes. When the temperature of the medium drops to about 30°C, add Bacillus licheniformis, Klebsiella indica, Bacillus thuringiensis, Paecilomyces lilacinus, gum The fermented seeds of Bacillus frozen-like were inoculated into sterilized fermenters respectively, and fermented for 30 hou...
Embodiment 2
[0043] The preparation method of the present invention's anti-cropping composite microbial bacterial agent comprises the following steps:
[0044] (1) Preparation of microbial inoculum:
[0045] 3mL of Bacillus licheniformis, 2mL of Klebsiella indica, 3mL of Bacillus thuringiensis, 1mL of Paecilomyces lilacinus, and 2mL of Bacillus jelly-like were cultured to obtain fermented seeds. Add 6kg of agar powder, 10kg of mannitol, 2kg of magnesium sulfate, 2kg of molasses, 3kg of beef extract, 2kg of sodium chloride, 0.15kg of potassium dihydrogen phosphate, and 0.6kg of ammonium molybdate in a 1000L fermenter, and the balance is water; Sterilize and culture at pH 7.3 in a fermenter at 115°C for 20 minutes. When the temperature of the medium drops to about 30°C, add Bacillus licheniformis, Klebsiella indica, Bacillus thuringiensis, Paecilomyces lilacinus, gum The fermented seeds of Bacillus frozen sample were inoculated into sterilized fermenters respectively, and fermented for 36 h...
Embodiment 3
[0050] The preparation method of the present invention's anti-cropping composite microbial bacterial agent comprises the following steps:
[0051] (1) Preparation of microbial inoculum:
[0052] 4 mL of Bacillus licheniformis, 3 mL of Bacillus indica, 3 mL of Bacillus thuringiensis, 1 mL of Paecilomyces lilacinus, and 1 mL of Bacillus jelly-like were cultured to obtain fermented seeds. Add 5kg of agar powder, 14kg of mannitol, 1kg of magnesium sulfate, 2kg of molasses, 4kg of beef extract, 1kg of sodium chloride, 0.15kg of potassium dihydrogen phosphate, and 0.6kg of ammonium molybdate in a 1000L fermenter, and the balance is water; pH 7.3 Sterilize and cultivate in a fermenter at 115°C for 20 minutes. When the temperature of the medium drops to about 30°C, add Bacillus licheniformis, Klebsiella indica, Bacillus thuringiensis, Paecilomyces lilacinus, gum The fermented seeds of Bacillus frozen sample were inoculated into sterilized fermenters respectively, and fermented for 36...
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