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Method for rapidly breeding fatsia japonica 'Variegata' through tissue culture

A technology of octagonal gold plate and tissue culture, which is applied in culture medium, horticultural methods, botanical equipment and methods, etc., can solve the problems of low reproduction coefficient and survival rate, and achieve a plant that meets a large number of needs, has a neat plant type, and has excellent traits Effect

Inactive Publication Date: 2019-12-06
SHANGHAI SHANGFANG LANDSCAPE PLANT INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The flower-leaf octagonal gold plate is propagated by cuttings and other methods, and the reproduction coefficient and survival rate are very low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0040] The present invention will be described in detail below in conjunction with specific embodiments.

[0041] A method for rapid propagation of flower-leaf star anise gold plate by tissue culture, comprising the following steps:

[0042] (1) Obtaining sterile materials

[0043] Collect the young stems with terminal buds on the female parent of the flower leaf star anise in March in spring, wash them, soak them successively with 75% alcohol and 1‰ mercuric chloride, rinse them with sterile water, and cut the terminal buds into pieces. Small sections, inoculated on the terminal bud induction medium;

[0044] (2) Differentiation and proliferation of buds

[0045] After the budlets were inoculated on the terminal bud induction medium for 15 days, the base of the budlets began to expand, and obvious callus tissue was visible on the 25th day, and after culturing for 30 days, the cut callus with budlets was put into the adventitious bud proliferation medium for cultivation;

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Abstract

The invention relates to a method for rapidly breeding fatsia japonica 'Variegata' through tissue culture. The method comprises the steps of (1) sterile material obtaining, (2) bud differentiation andproliferation, (3) rooting culture and (4) seedling exercising and transplantation. Compared with the prior art, the rapid breeding method of tissue culture is adopted, and a large number of plants which are neat in plant type and excellent in trait can be obtained in a short period of time, so that the heavy demand in the market is met.

Description

technical field [0001] The invention relates to a plant tissue culture and breeding method, in particular to a method for tissue culture and fast breeding of star anise gold plate. Background technique [0002] Fatsia japonica 'Variegata' (scientific name: Fatsia japonica 'Variegata') is an evergreen shrub or small tree plant in the Araliaceae family, with a plant height of up to 5 meters. Smooth without thorns. The petiole is 10-30cm long; the leaf blade is large, leathery, nearly round, with yellow or white spots on the leaf surface, 12-30cm in diameter, palmately 7-9 deeply lobed, lobes oblong-ovate, apex short and acuminate, Heart shaped base. Panicle terminal, 20-40 cm long; umbel 3-5 cm in diameter, inflorescence axis covered with brown tomentose; calyx nearly entire, glabrous; petals 5, ovate-triangular, 2.5-3 mm long, yellow-white, Glabrous; stamens 5, filaments as long as petals; ovary inferior, 5-chambered, with 1 blastule in each chamber; styles 5, separated; d...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00A01G24/28A01G24/15
CPCA01H4/001A01H4/008A01G31/00A01G24/28A01G24/15
Inventor 李秋静林丹卿霞周天宇唐忠炳张文昊庞玉华秦祥
Owner SHANGHAI SHANGFANG LANDSCAPE PLANT INST
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