A kind of method for extracting soybean isoflavones from soymilk water primary scum
A technology of soybean isoflavones and soybean milk, applied in the direction of organic chemistry, etc., to achieve the effects of reduced usage, short extraction time, and high extraction efficiency
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Embodiment 1
[0066] 1) Take 100g of first-class scum powder and add absolute ethanol to 700mL, finely extract it with a colloid mill for 15 minutes, and rinse the colloid mill with 300mL. Filtrate 900mL, add absolute ethanol to the filter residue to 700mL, grind the colloid mill for 40min, rinse the colloid mill 300mL, and filtrate 900mL.
[0067] 2) The two filtrates were combined, rotary evaporated to dryness to obtain 2.5 g of paste, 25 mL of n-hexane was added to the paste, vortex extracted for 30 min, centrifuged at 1500 r / min for 8 min, and the supernatant was removed to obtain slag.
[0068] 3) Add 30 mL of ethyl acetate to the slag, vortex extract for 30 min, centrifuge at 1500 r / min for 15 min, take the supernatant, and spin evaporate to dryness to obtain 326.9 mg of soybean isoflavones with a purity of 94.5%.
Embodiment 2
[0070] 1) Take 100g of first-grade scum powder and add absolute ethanol to 1000mL, finely extract it with a colloid mill for 20min, and rinse the colloid mill with 500mL. Filtrate 1300mL, add absolute ethanol to the filter residue to 1000mL, grind the colloid mill for 80min, rinse the colloid mill 500mL, and filtrate 1300mL.
[0071] 2) The two filtrates were combined, rotary evaporated to dryness to obtain 2.85 g of paste, 30 mL of n-hexane was added to the paste, vortex extracted for 30 min, centrifuged at 2000 r / min for 5 min, and the supernatant was removed to obtain slag.
[0072] 3) Add 30 mL of ethyl acetate to the slag, vortex extract for 20 min, centrifuge at 2000 r / min for 10 min, take the supernatant, and spin evaporate to dryness to obtain 335.2 mg of soybean isoflavones with a purity of 93.9%.
Embodiment 3
[0074] 1) Take 100g of first-grade scum powder and add absolute ethanol to 800mL, finely extract it with a colloid mill for 20 minutes, and rinse the colloid mill with 500mL. Filtrate 1100mL, add absolute ethanol to the filter residue to 800mL, grind the colloid mill for 60min, rinse the colloid mill 600mL, and filtrate 1200mL.
[0075]2) The two filtrates were combined, rotary evaporated to dryness to obtain 2.83g paste, 30mL of n-hexane was added to the paste, vortex extracted for 20min, centrifuged at 1000r / min for 10min, and the supernatant was removed to obtain slag.
[0076] 3) Add 25 mL of ethyl acetate to the slag, vortex extract for 325 min, centrifuge at 1000 r / min for 20 min, take the supernatant, and spin evaporate to dryness to obtain 331.8 mg of soybean isoflavones with a purity of 94.3%.
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