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A kind of method for extracting soybean isoflavones from soymilk water primary scum

A technology of soybean isoflavones and soybean milk, applied in the direction of organic chemistry, etc., to achieve the effects of reduced usage, short extraction time, and high extraction efficiency

Active Publication Date: 2021-06-01
SHANDONG YUWANG ECOLOGY FOOD IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is not easy to extract high-purity soybean isoflavones

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] 1) Take 100g of first-class scum powder and add absolute ethanol to 700mL, finely extract it with a colloid mill for 15 minutes, and rinse the colloid mill with 300mL. Filtrate 900mL, add absolute ethanol to the filter residue to 700mL, grind the colloid mill for 40min, rinse the colloid mill 300mL, and filtrate 900mL.

[0067] 2) The two filtrates were combined, rotary evaporated to dryness to obtain 2.5 g of paste, 25 mL of n-hexane was added to the paste, vortex extracted for 30 min, centrifuged at 1500 r / min for 8 min, and the supernatant was removed to obtain slag.

[0068] 3) Add 30 mL of ethyl acetate to the slag, vortex extract for 30 min, centrifuge at 1500 r / min for 15 min, take the supernatant, and spin evaporate to dryness to obtain 326.9 mg of soybean isoflavones with a purity of 94.5%.

Embodiment 2

[0070] 1) Take 100g of first-grade scum powder and add absolute ethanol to 1000mL, finely extract it with a colloid mill for 20min, and rinse the colloid mill with 500mL. Filtrate 1300mL, add absolute ethanol to the filter residue to 1000mL, grind the colloid mill for 80min, rinse the colloid mill 500mL, and filtrate 1300mL.

[0071] 2) The two filtrates were combined, rotary evaporated to dryness to obtain 2.85 g of paste, 30 mL of n-hexane was added to the paste, vortex extracted for 30 min, centrifuged at 2000 r / min for 5 min, and the supernatant was removed to obtain slag.

[0072] 3) Add 30 mL of ethyl acetate to the slag, vortex extract for 20 min, centrifuge at 2000 r / min for 10 min, take the supernatant, and spin evaporate to dryness to obtain 335.2 mg of soybean isoflavones with a purity of 93.9%.

Embodiment 3

[0074] 1) Take 100g of first-grade scum powder and add absolute ethanol to 800mL, finely extract it with a colloid mill for 20 minutes, and rinse the colloid mill with 500mL. Filtrate 1100mL, add absolute ethanol to the filter residue to 800mL, grind the colloid mill for 60min, rinse the colloid mill 600mL, and filtrate 1200mL.

[0075]2) The two filtrates were combined, rotary evaporated to dryness to obtain 2.83g paste, 30mL of n-hexane was added to the paste, vortex extracted for 20min, centrifuged at 1000r / min for 10min, and the supernatant was removed to obtain slag.

[0076] 3) Add 25 mL of ethyl acetate to the slag, vortex extract for 325 min, centrifuge at 1000 r / min for 20 min, take the supernatant, and spin evaporate to dryness to obtain 331.8 mg of soybean isoflavones with a purity of 94.3%.

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Abstract

The disclosure provides a method for extracting soybean isoflavones from the primary scum of soybean milk water. The method is as follows: the primary scum of soybean milk water is extracted with absolute ethanol, degreased with n-hexane, and purified with ethyl acetate to obtain soybean isoflavones ; or, the first-class scum of soybean milk is extracted with ethanol, rectified, adsorbed and eluted by macroporous resin, and dried to obtain soybean isoflavone crystals. The present disclosure can extract soybean isoflavones from the primary scum of soybean milk water.

Description

technical field [0001] The disclosure relates to a method for extracting soybean isoflavones from primary scum of soybean milk water. Background technique [0002] The statements herein merely provide background information related to the present disclosure and may not necessarily constitute prior art. [0003] Soybean belongs to Dicotyledoneae, Fabaceae, Papilionaceae, and is very rich in protein, about 40% in dry beans, which is rich in 8 kinds of amino acids that cannot be synthesized by the human body. Many studies in recent years have shown that soybean isoflavones have a variety of physiological functions, so the demand for soybean isoflavones is gradually increasing. Soy isoflavones are usually solid, with a melting point above 100°C. They are stable at room temperature. They are yellowish-white, powdery, non-toxic, and have a slightly bitter taste. They have a certain Solubility, insoluble in cold water, easily soluble in hot water, insoluble in petroleum ether, n-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/36C07D311/40
CPCC07D311/36C07D311/40
Inventor 刘汝萃王笛鲁绪强侯尔群韩亮董欣伟李成辉刘代成
Owner SHANDONG YUWANG ECOLOGY FOOD IND
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