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Real-time monitoring cell culture system and culture method thereof

A technology for cell culture and real-time monitoring, which is applied in the fields of biology and cell culture, and can solve problems such as increasing operating time for operators, increasing the risk of medium contamination, and affecting the stability of the culture environment

Active Publication Date: 2019-12-10
ZHUJIANG HOSPITAL SOUTHERN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, frequent opening will affect the stability of the culture environment, and will increase the risk of contamination of the medium and increase the operating time of the operator

Method used

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  • Real-time monitoring cell culture system and culture method thereof
  • Real-time monitoring cell culture system and culture method thereof
  • Real-time monitoring cell culture system and culture method thereof

Examples

Experimental program
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Embodiment Construction

[0028] Embodiments of the application are described in detail below, examples of which are illustrated in the accompanying drawings. The embodiments described below by referring to the figures are exemplary only for explaining the present application, and are not construed as limiting the present application. In order to demonstrate the practical application of the real-time monitoring cell culture system, the following examples will introduce the specific operation steps of the method, so that the application and effect of the real-time monitoring cell culture system can be more fully and easily understood. It should be noted that the protection scope of this application is not limited.

[0029] Please refer to figure 1 , figure 1 Demonstrate a real-time monitoring cell culture system 1, in order to demonstrate the convenience and simplify the structure of the display, figure 1 The pressurization device, air conditioning device, opening and closing device, rotating motor a...

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Abstract

The invention provides a real-time monitoring cell culture system in order to observe the internal environment of a cell culture device under the condition of maintaining the stability of a cell culturing microenvironment. The real-time monitoring cell culture system comprises an image collection device which is used for collecting images of cells in the culture system, a cell culture device whichcomprises a cell culture cavity for culturing cells and moves relatively to the image collection device, and a rotary transmission device which is used for providing power to relatively circumferential motion between the image collection device and the cell culture device. According to the technical scheme, the cell culture system enables a user to observe the status of cell culture without opening the cell culture cavity, is favorable to maintaining the balance of the cell culturing microenvironment, can be used for reducing the workload of the user for frequently opening or closing the cellculture cavity, is favorable to reducing the risk of bacterium invasion in the cell culturing process, and can be used for increasing the survival rate of cell culture.

Description

technical field [0001] This application mainly relates to the fields of biology and cell culture, in particular to a real-time monitoring cell culture system and its culture method. Background technique [0002] Cell culture is an important means of scientific research and production. Cell culture has relatively high requirements for equipment and requires close observation. The current cell culture is mainly cultivated under constant temperature and humidity conditions, often relying on equipment such as constant temperature and humidity chambers. During the process of culturing cells, it is often necessary to observe the growth status of cells for recording or as a basis for adjusting culture conditions, etc. state. [0003] In existing cell culture devices, especially in pressurized devices, taking out cultured cells affects the atmosphere of cultured cells to a certain extent, such as temperature and humidity, carbon dioxide content, and the like. At the same time, fre...

Claims

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Application Information

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IPC IPC(8): C12M3/02C12M1/34C12M1/12C12M1/02
CPCC12M23/48C12M23/52C12M27/02C12M29/04C12M29/06C12M29/18C12M41/12C12M41/18C12M41/44C12M41/46
Inventor 李阳高毅钟克波彭青
Owner ZHUJIANG HOSPITAL SOUTHERN MEDICAL UNIV
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