Gel preparation device for SDS-PAGE (sodium dodecylsulfate-polyacrylamide gel electrophoresis)

A preparation device and gel technology, which is applied in the field of gel preparation devices, can solve problems such as time-consuming, rework, and unavoidable solidification time, and achieve the effects of saving waiting time and preventing glue leakage

Pending Publication Date: 2019-12-20
SHANDONG FOREST GERMPLASM RESOURCES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the separating gel and stacking gel in the discontinuous buffer system need to be configured sequentially, and it takes a long time to solidify after configuration.
Because the gel can only achieve a better separation effect if it is fully solidified, the coagulation time is inevitable. At present, other tasks are mainly arranged through the coagulation time to improve the time utilization rate, and the coagulation can also be accelerated by increasing the temperature, but accelerating coagulation will cause separation. not effectively
[0003] In addition, if the gel device is not assembled tightly, there will be glue leakage, and reconfiguration after glue leakage will also waste time
Glue leakage is the phenomenon that the glue flows out through the gap before solidification under the action of gravity, because the gap is generally not too large, the glue flows out slowly, and in addition to the existence of solidification, the glue usually only flows out a small part, But even a small outflow will inevitably lead to rework

Method used

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  • Gel preparation device for SDS-PAGE (sodium dodecylsulfate-polyacrylamide gel electrophoresis)
  • Gel preparation device for SDS-PAGE (sodium dodecylsulfate-polyacrylamide gel electrophoresis)
  • Gel preparation device for SDS-PAGE (sodium dodecylsulfate-polyacrylamide gel electrophoresis)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Such as figure 1 , 2 , 3, a gel preparation device for SDS-PAGE, comprising a back plate 1 and a front plate 2, spacers 11 are respectively set on both sides of the back plate 1, gaps 12 are set on the spacers 11, and the The front board 2 is made up of a front upper board 21 and a front lower board 22 .

[0052] Further, two spacers 11 are arranged in parallel.

[0053] Further, the gap 12 divides the spacer 11 into an upper spacer 111 and a lower spacer 112 .

[0054] Further, after the rear panel 1 and the front panel 2 are assembled, the notch 12 corresponds to the gap between the front upper panel 21 and the front lower panel 22 .

[0055] Further, a notch 23 is provided on the upper edge of the front upper plate 21 , and plate ears 24 are formed on both sides.

Embodiment 2

[0057] On the basis of Example 1, as Figure 4 As shown, a sealing strip 3 is also included, and the sealing strip 3 is connected as a whole by a bottom sealing strip 31 , a side sealing strip 32 and a middle sealing strip 33 .

[0058] Further, the sealing strip 3 is used to seal the gap between the rear panel 1 and the front panel 2, so as to ensure that the glue will not leak out during the glue making process.

[0059] Further, the bottom sealing strip 31 is used to seal the gap between the bottom of the front lower panel 22 and the rear panel 1 , and the side sealing strip 32 is used to seal the rear panel 1 and the front panel 2 The gap between the two sides, the middle sealing strip 33 is used to block the gap between the bottom of the front upper board 21 and the rear board 1 .

Embodiment 3

[0061] On the basis of Example 2, such as Figure 5 As shown, the section of the middle seal 33 is "L" or "⊥" type, and the positional relationship in the gel device is as attached Figure 5 shown.

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Abstract

The invention discloses a gel preparation device for SDS-PAGE (sodium dodecylsulfate-polyacrylamide gel electrophoresis). The device comprises a rear plate and a front plate, wherein spacing pieces are respectively arranged on both sides of the rear plate; notches are formed in the spacing pieces; and the front plate consists of an upper front plate and a lower front plate. By adopting the device,the time for waiting separation gel to be cured is avoided, the assembling and sample dispensing time of an electrophoresis device is reasonably utilized, and in addition, the probability of gel leakage can be reduced to a certain extent.

Description

technical field [0001] The invention relates to a gel preparation device for SDS-PAGE. Background technique [0002] Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is an important means to separate proteins according to the molecular weight of protein subunits. SDS-PAGE generally uses a discontinuous buffer system, which can have higher resolution than a continuous buffer system. However, in the discontinuous buffer system, the separating gel and the stacking gel need to be prepared sequentially, and it takes a long time to solidify after the preparation. Because the gel can only achieve a better separation effect if it is fully solidified, the coagulation time is inevitable. At present, other tasks are mainly arranged through the coagulation time to improve the time utilization rate, and the coagulation can also be accelerated by increasing the temperature, but accelerating coagulation will cause separation. not effectively. [0003] In addition, if...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/00G01N27/447C07K1/26C08J3/02C08L33/26C08K5/42
CPCB01J13/0065C07K1/26C08J3/02C08J2333/26C08K5/42G01N27/447
Inventor 李文清王磊赵永军于梅韩庆军吴丹
Owner SHANDONG FOREST GERMPLASM RESOURCES CENT
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