Ultrasonic uniform mixing method for micro-fluidic chip reagents

A microfluidic chip and ultrasonic technology, applied in chemical instruments and methods, laboratory utensils, laboratory containers, etc., can solve the problem of undisclosed microfluidic chip pretreatment, reduce reagent residue or loss, The effect of improving utilization and short time

Active Publication Date: 2019-12-20
HUNAN LEGEND AI CHIP BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Prior art, such as patent publication number: CN207730782U, patent name: a sample reagent tray; patent publication number: CN106483007A, patent name: mixer and a mixing device using the mixer, patent publication number: CN208526495U, patent Name: Ultrasonic mixing devices, all of which use ultrasonic mixing after direct contact with the reagents, and none of them discloses the technical scheme of using intermittent ultrasound to pretreat the reagents of the microfluidic chip

Method used

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  • Ultrasonic uniform mixing method for micro-fluidic chip reagents
  • Ultrasonic uniform mixing method for micro-fluidic chip reagents
  • Ultrasonic uniform mixing method for micro-fluidic chip reagents

Examples

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Effect test

Embodiment 1

[0056] Magnetic bead suspensions are used in chemiluminescent immunoassays or molecular diagnostic nucleic acid extraction processes. Although the types and particle sizes of magnetic beads are different, they are all composed of solid magnetic bead particles and diluents. After being stored in the chip, Sedimentation occurs on standing (eg figure 2 shown). If the deposited magnetic bead suspension participates in related reactions, the stability and reliability of the test results will be seriously affected. Therefore, in order to ensure accurate and reliable test results, the magnetic bead suspension must be mixed before use to resuspend and disperse the magnetic beads evenly. Therefore, it is necessary to perform continuous ultrasonic period A' to fully mix the magnetic beads; in addition, the magnetic bead liquid may splash on the plunger during the execution of A', causing the plunger to stick to a small section of reagent, which needs to be shaken back to the bottom aga...

Embodiment 2

[0077] Microfluidic chips will inevitably be bumped or even turned upside down during transportation, and a small amount of air bubbles may be mixed in the reagents. Due to the small diameter of the reagent storage chamber, a small piece of reagent may be attached to the plunger, and the liquid column may be stratified up and down (such as Figure 4 As shown), the reagent cannot fall back to the bottom of the reagent storage chamber smoothly only by its own gravity. The existence of air bubbles in the reagent or the problem of reagent segmentation may cause inaccurate volumes of reagents added to the reaction area, or it is necessary to add more reagents to the reagent storage chamber to reduce air, resulting in waste of reagents, reduced reagent utilization, and significantly increased testing costs. Because the reagent itself is a homogeneous solution in this example, it is not necessary to perform the continuous ultrasonic period A' for vigorous mixing, and only need to per...

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Abstract

The invention provides an ultrasonic uniform mixing method for micro-fluidic chip reagents. The method comprises that an ultrasonic probe emits intermittent ultrasonic waves to reagent storage cavities in a micro-fluidic chip so as to oscillate reagents, wherein the intermittent ultrasonic treatment comprises alternately circulating a plurality of ultrasonic time periods A2 and a plurality of non-ultrasonic time periods B2, (A2+B2)*N2 is equal to C2, N2 is the total number of the circulation times of the ultrasonic time periods A2 and the non-ultrasonic time periods B2, C2 is the intermittentultrasonic uniform mixing working time, the ultrasonic probe stops the emitting of the ultrasonic waves after the ultrasonic uniform mixing working time C2 is finished, the duration of the non-ultrasonic time period B2 is five times or more the duration of the ultrasonic time period A2;, and the ultrasonic time period A2 in each cycle does not exceed 0.1 s. Compared with the method in the prior art, by using the intermittent working mode, through the alternate circulation of the ultrasonic time periods and the non-ultrasonic time periods with different time lengths, the sufficient and uniformmixing of the reagents in the reagent storage cavities and the gas bubble eliminating are achieved.

Description

technical field [0001] The invention relates to the technical field of biochip testing, in particular to a method for ultrasonically mixing microfluidic chip reagents. Background technique [0002] As a disposable consumable, the microfluidic biochip in the field of analysis and detection must pre-seal all the reagents required for the test project inside the chip. Several reagent storage cavities are provided in the microfluidic chip, and various reagents are respectively provided in the several reagent storage cavities. A reagent storage chamber can store 50 microliters of reagents. The reagent storage chamber has a columnar structure, and a plunger is arranged on the top to isolate the reagent storage chamber from the external space of the chip. The bottom of the reagent storage chamber is provided with a microfluidic pipeline with a microvalve leading to the reaction area. During the test, the microvalve is opened, and the plunger is pushed by mechanical external forc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00
CPCB01L3/5027B01L2200/16
Inventor 邬鹏程黄常青高飞
Owner HUNAN LEGEND AI CHIP BIOTECH CO LTD
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