A kind of propolis nanoparticle, preparation method, application and preparation method of freeze-dried powder thereof
A nanoparticle and freeze-dried powder technology, applied in nano-drugs, freeze-dried delivery, nanotechnology and other directions, can solve the problems of low oral absorption and bioavailability, difficult practical application, etc., achieve good industrialization prospects and enhance application value. Effect
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Embodiment 1
[0049] The preparation method of propolis nanoparticles, the steps are: (1) take 5 mg of TPGS and dissolve it in 5 ml of water under ultrasonic conditions as the water phase; (2) weigh 10 mg of propolis and completely dissolve it in 0.2 ml of ethanol to obtain an organic phase; (3) Under 250W ultrasonic conditions, the organic phase obtained in the step (2) is slowly added dropwise in the aqueous phase obtained in the step (1), to obtain a mixed phase; (4) the mixed phase obtained in the step (3) at 45 Propolis nanoparticles were obtained by rotary evaporation under reduced pressure at ℃ to remove all ethanol.
[0050] The average particle diameter of the obtained propolis nanoparticles was measured to be 131.3nm, the PDI value was 0.287, and the surface potential was -25.5mV. After standing at room temperature for 5 days, the average particle diameter was 122.2nm, the PDI value was 0.246, and the surface potential was -28.5mV.
Embodiment 2
[0052] The preparation method of propolis nanoparticles, the steps are: (1) take 2mg TPGS and dissolve it in 5ml water under ultrasonic conditions as the water phase; (2) weigh 10mg propolis and dissolve it completely in 0.2ml ethanol to obtain the organic phase; (3) Under 250W ultrasonic conditions, the organic phase obtained in the step (2) is slowly added dropwise in the aqueous phase obtained in the step (1), to obtain a mixed phase; (4) the mixed phase obtained in the step (3) at 45 Rotary evaporation under reduced pressure at ℃, after removing all ethanol, propolis nanoparticles were obtained. The average particle diameter of the obtained propolis nanoparticles was measured to be 177.4nm, the PDI value was 0.251, and the surface potential was -33.0mV.
Embodiment 3
[0054] The preparation method of propolis nanoparticles, the steps are: (1) take 2.5mgTPGS and dissolve it in 1ml water under ultrasonic conditions as the water phase; (2) weigh 10mg propolis and dissolve it completely in 0.2ml ethanol to obtain the organic phase; (3) Under 250W ultrasonic conditions, slowly drop the organic phase obtained in step (2) into the water phase to obtain a mixed phase; (4) Rotate the mixed phase under reduced pressure at 45°C, remove all ethanol, Centrifuge for 5 min at a rotating speed of 1 / min to remove impurities to obtain propolis nanoparticles. The average particle diameter of the obtained propolis nanoparticles was measured to be 169.5nm, the PDI value was 0.254, and the surface potential was -10.2mV.
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