Method for disintegrating human pluripotent stem cells into macrophages

A macrophage, volume percentage technology, applied in the biological field, can solve problems such as hindering clinical application, long differentiation cycle, and low efficiency
CN110607277AActive Publication Date: 2019-12-24TSINGHUA UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TSINGHUA UNIV
Publication Date
2019-12-24

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Abstract

The invention discloses a method for disintegrating human pluripotent stem cells into macrophages. The invention provides a reagent kit for disintegrating the pluripotent stem cells into the macrophages. The reagent kit comprises a complete set of culture fluid which can disintegrate the pluripotent stem cells into the macrophages. A monolayer cell culture method is adopted, firstly, hemutopoiesisstem cells are obtained through disintegrating, and then through further adding IL3 and M-CSF for stimulation, CD14 and CD163 positive macrophages are obtained. The method has the advantages that chemical components are definite, animal-source components do not exist, the safety of preparation cells is greatly increased, and the method has the characteristics of short in time consumption, high indisintegrating efficiency, low in cost and the like. Through the adoption of the preparation method provided by the invention, human macrophages can be produced in a large-scale manner, the quality is stable, the safety is high, and massive cell sources are provided for tissue project, medicine research and development and cell therapy.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a method for differentiating macrophages from human pluripotent stem cells. Background technique

[0002] Human pluripotent stem cells include human embryonic stem cells and human induced pluripotent stem cells, which can differentiate into various types of cells in the human body. They can be used to make disease models, conduct drug toxicity tests, and replace damaged and diseased cells through cell transplantation to promote Body wound repair and disease treatment.

[0003] Macrophages are important natural immune cells of the human body. They have important application value in resisting pathogenic microbial infection, removing necrotic cells and tissue debris, and studying the occurrence and development of atherosclerosis. At present, it is difficult to obtain a large number of human macrophages, and it is difficult to expand and genetically manipulate, which greatly limits rel...

Claims

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