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Microscope camera light source discrimination method and self-adaptive white balance method and device for microscope camera

A discrimination method and microscope technology, applied in microscopes, optics, optical components, etc., can solve the problems of not being concise enough, unable to meet the needs of accurate imaging of multi-light source microscopic platforms, and unable to meet the needs of accurate imaging of multi-light source microscopic platforms, to achieve The results are accurate

Active Publication Date: 2019-12-24
杭州图谱光电科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A single white balance algorithm can no longer meet the needs of accurate imaging of multi-light source microscope platforms, or the algorithm library is complex and not streamlined enough, or simple but not applicable
[0009] The white balance adjustment methods disclosed in the application publication numbers CN103795992A and CN103780892A are general white balance methods. When the light sources are different, the general white balance methods cannot meet the needs of accurate imaging of multi-light source microscope platforms

Method used

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  • Microscope camera light source discrimination method and self-adaptive white balance method and device for microscope camera
  • Microscope camera light source discrimination method and self-adaptive white balance method and device for microscope camera

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] This embodiment provides a microscope camera light source discrimination method, including the following steps:

[0057] S101. Construct the corresponding relationship between the microscope light source and the smallest circumscribed circle and the center and radius of the smallest circumscribed circle.

[0058] In this embodiment, a photoelectric image sensor IMX183 is used to collect microscopic images. Specifically, different light sources are used to irradiate the white object, through the optical path, that is, the microscope imaging, and then through the interface between the microscope and the camera, the image is collected by the 16-megapixel camera whose sensor is IMX183, and then the image data is input into HiSilicon’s Hi3519A chip , perform preliminary screen clipping, analyze RGB data, and wait for further processing.

[0059] The G component normalization is performed on the white object images collected under the illumination of each light source, that ...

Embodiment 2

[0066] On the basis of Example 1, in order to solve the problem of inaccurate color reproduction when the microscope light source emits obviously yellowish or bluish light, this example is based on its own rich theoretical knowledge of image processing and in-depth understanding of various mainstream microscopes , according to the basic white balance principle, through a large number of tests and simulations, a more extensive and accurate white balance method for automatically matching different color temperature light sources for microscope cameras is designed.

[0067] Specifically, as figure 1 As shown, the adaptive white balance method for a microscope camera provided in this embodiment includes the following steps:

[0068] S201. Construct the corresponding relationship between the microscope light source and the smallest circumscribed circle and the center and radius of the smallest circumscribed circle.

[0069] Step S201 is basically the same as step 101, using the ph...

Embodiment 3

[0085] On the basis of Embodiments 1 and 2, in order to solve the problem of inaccurate color reproduction when the microscope light source emits obviously yellowish or bluish light, this embodiment proposes an adaptive white balance device for a microscope camera, specifically including:

[0086] a computer memory, a computer processor, and a computer program stored in said computer memory and executable on said computer processor,

[0087] The position of the minimum circumscribed circle corresponding to each type of microscope light source determined by the microscope camera light source discrimination method provided in embodiment 1 is stored in the computer memory;

[0088] When the computer processor executes the computer program, the method for adaptive white balance for a microscope camera provided in Embodiment 2 is implemented, that is, when the computer processor executes the computer program, the following steps are performed:

[0089] S301. Determine the microscop...

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Abstract

The invention discloses a microscope camera light source discrimination method. According to the microscope camera light source discrimination method, the type of a microscope camera light source canbe determined according to the distribution state of pixel points in an rb space. The invention also discloses a self-adaptive white balance method and device for the microscope camera, and the methodand the device employ corresponding white balance correction strategies for different light source types after employing a microscope camera light source discrimination method to discriminate the light source types, and can achieve the accurate white balance calibration of an image.

Description

technical field [0001] The invention belongs to the field of microscopic imaging, and in particular relates to a method for discriminating a light source of a microscope camera, and an adaptive white balance method and device for a microscope camera. Background technique [0002] A microscope is an optical device used to observe the microstructure of microorganisms, cells or some substances. [0003] The digital camera dedicated to the microscope uses the photoelectric image sensor to capture the image formed by the microscope system, then processes the image through the ISP (image processor) inside the chip, and finally displays it on the display for observation or saves it for subsequent analysis and processing. [0004] An optical microscope is mainly composed of a light source, a mirror, a stage, an objective lens and an eyepiece. The function of the light source is to provide illumination for the microscope. The light sources of existing optical microscopes generally ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): H04N9/73H04N17/00H04N17/02G02B21/06
CPCH04N17/002H04N17/02G02B21/06H04N23/88
Inventor 余飞鸿何权奇程亮
Owner 杭州图谱光电科技有限公司