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A method for separating and purifying deacetoxycephalosporin C from cephalosporin C fermentation broth

A kind of technology of deacetoxy head, cephalosporin, applied in the field of separation and purification

Active Publication Date: 2020-08-04
LIVZON NEW NORTH RIVER PHARMA
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] At present, there is no relevant report on the purification and separation of DOCPC. How to separate and purify DOCPC from cephalosporin C fermentation broth is difficult and has very important practical significance.

Method used

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  • A method for separating and purifying deacetoxycephalosporin C from cephalosporin C fermentation broth
  • A method for separating and purifying deacetoxycephalosporin C from cephalosporin C fermentation broth
  • A method for separating and purifying deacetoxycephalosporin C from cephalosporin C fermentation broth

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Embodiment 1

[0041] A method for separating and purifying deacetoxycephalosporin C from cephalosporin C fermentation liquid, comprising the steps of:

[0042] 1) Take a certain amount of cephalosporin C fermentation broth, and concentrate it under reduced pressure with a rotary evaporator (water bath temperature 40° C.) to 1 / 6 to 1 / 4 of the original volume to obtain a cephalosporin C concentrated solution;

[0043] 2) The concentrated solution of cephalosporin C is subjected to high-pressure liquid chromatography preparative column processing, which specifically includes the following steps:

[0044] Ⅰ) The cephalosporin C concentrated solution is subjected to the first high-pressure liquid chromatography preparative column treatment, and the high-pressure liquid chromatography column treatment conditions are as follows: a C18 packing chromatographic preparative column is used, the packing is Peace ODS, the mobile phase A is acetonitrile, and the mobile phase B is 0.1% formic acid aqueous ...

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Abstract

The invention discloses a method for separating and purifying deacetoxycephalosporin C from cephalosporin C fermentation liquor. The method comprises the following steps: 1) taking the cephalosporin Cfermentation liquor, and concentrating to obtain a cephalosporin C concentrated solution; and 2) carrying out liquid chromatography preparation column separation on the cephalosporin C concentrated solution to obtain the deacetoxycephalosporin C. According to the method, the DOCPC is separated from the cephalosporin C fermentation liquor for the first time; in addition, the purity of the DOCPC obtained through separation and purification is 97% or above; therefore, the high-purity DOCPC can be effectively separated and purified from the cephalosporin C fermentation liquor, a reliable foundation is provided for pharmacological research of the DOCPC, meanwhile, a foundation is laid for further obtaining the high-purity DOCPC, and the method has quite important practical significance.

Description

technical field [0001] The invention relates to the field of separation and purification, in particular to a method for separating and purifying deacetoxycephalosporin C from cephalosporin C fermentation liquid. Background technique [0002] Cephalosporins are members of the β-lactam antibiotic family, and currently account for 51% of my country's antibiotic drug market, and penicillins account for about 17%. The biosynthesis of cephalosporin C (CPC) is similar to that of penicillin. In order to determine the end point of fermentation and feed properly to control the fermentation process, it is necessary to effectively detect the composition of the medium, early impurities, and the ratio of fermentation products and by-products during the fermentation process. [0003] Cephalosporin C fermentation process includes CPC, deacetyl cephalosporin C (DCPC), deacetoxy cephalosporin C (DOCPC) and other substances, deacetoxy cephalosporin C (DOCPC) is a cephalosporin C (CPC) is a by...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D501/28C07D501/12
CPCC07D501/12C07D501/28
Inventor 张勇陈倩倩王珂黄恩贤李想唐阳刚
Owner LIVZON NEW NORTH RIVER PHARMA
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