Wall breaking method of chlorella

Abstract

The invention relates to a wall breaking method of chlorella. The wall breaking method comprises the following steps: dissolving chlorella dried powder in water in a 10g / L material-liquid ratio to obtain a chlorella liquid; adding cellulase which is 1-2 percent of the mass of the cellulase dried powder into the cellulase liquid, and regulating the pH of the cellulase liquid to be 4-5; adding the pH regulated cellulase liquid into a colloid mill, and performing grinding treatment at a rotating speed of 8000-10000r / min for 1 hour; putting the ground cellulase liquid in water bath at 45-50 DEG C,and performing stirring, heating and enzymolysis for 2-3 hours so as to complete the wall breaking treatment for chlorella. The method can perform enzyme wall breaking by utilizing colloid mill equipment and disperse the caking phenomenon of the chlorella powder through the colloid mill, has the effect of grinding and pulverizing cell walls, can achieve a relatively high wall breaking rate for chlorella with a small amount of enzyme and a relatively short time, is low in cost and simple in operation.

Description

technical field [0001] The invention relates to the technical field of algae treatment, in particular to a method for breaking the wall of chlorella. Background technique [0002] Chlorella sp is a kind of universal unicellular green algae, which belongs to Oocystaceae Chlorophyta Chlorophyceae and has been widely cultivated. The algae powder of chlorella grown under good conditions has high application value, the protein content can reach 63.60%, and it also contains docosahexaenoic acid (DHA). In the United States and Japan, Chlorella has been used as an excellent food and animal feed additive for more than 30 years. In recent years, my country has also begun to attach importance to the development and utilization of chlorella. However, because chlorella has a tough cell wall, it cannot be directly eaten, and the extraction of functional substances such as growth factors (CGF) in it is also hindered, so that the wide utilization of chlorella is limited to a certain exten...

AI Technical Summary

Problems solved by technology

However, because chlorella has a tough cell wall, it cannot be eaten directly, and the extraction of its internal functional substances such as growth factors (CGF) is also hindered, causing the widespread utilization of chlorella to be limited to a certain extent.

[0003] At present, the wall-breaking methods of Chlorella mainly include physical wall-breaking methods and biochemical wall-breaking methods; physical wall-breaking methods include grinding methods, mechanical homogenization methods, ultrasonic wall-breaking methods, and repeated freeze-thaw methods. The time is long, and the efficiency of wall breaking is low; the efficiency of ultrasonic wall breaking is high, but it has high requirements for the concentration of broken algae cells. Scale breaking

However, the enzymatic wall-breaking method in the biochemical wall-breaking method cannot deal with the agglomeration of algae cells, which affects the wall-breaking rate, making it necessary to add a large amount of enzymes for treatment or prolong the time of enzymatic treatment, resulting in an increase in the cost of wall-breaking. low efficiency

[0004] Therefore, the current wall-breaking method for extracting Chlorella still needs to be improved