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A method for the semi-continuous production of gentamicin c1a based on the control of specific growth rate
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A technology of gentamicin and growth rate, applied in the direction of bacteria, fermentation, etc., can solve the problem of low specific growth rate and achieve the effect of improving production efficiency
Active Publication Date: 2021-12-07
QINGDAO INNOVATION INST OF EAST CHINA UNIV OF SCI & TECH +1
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Problems solved by technology
[0004] However, there are not many related studies on the relationship between specific growth rate and specific product production rate
Method used
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Embodiment 1
[0025] Prepare primary seed medium, secondary seed medium, fermentation medium and feed medium according to the ratio described in the summary of the invention, and then sterilize with high pressure steam for use.
[0026] The components and mass percentages of the fermented feed medium used to feed the fermented liquid are as follows: glucose 10%, soybean powder 8%, ammonium sulfate 3.0%, potassium sulfate 1.0%, potassium nitrate 0.9%, calcium carbonate 0.6%, chlorine Cobalt oxide 0.005%, calcium oxide 0.1%, foam enemy 0.3%, corn steep liquor 1.0%, the rest is water, and the pH is adjusted to 7.0±0.5 before elimination.
[0027] The medium used for the primary seed culture is a primary seed medium, and its components and mass percentages are as follows: 3.0% starch, 5.0% corn flour, 4.5% soybean flour, 1.2% glycine, 3.0% ammonium sulfate, 0.8% potassium sulfate %, potassium nitrate 0.7%, calcium carbonate 1.2%, calcium oxide 0.07%, foam enemy 0.2%, the rest is water, adjust t...
Embodiment 2
[0037] Others are all the same as in Example 1, the difference is that feeding starts when the capacitance value reaches 10pf, and the total flow rate of feeding is F=0.25L / h, wherein the ratio of feeding and water replenishment is adjusted according to the changing trend of the capacitance value to maintain the capacitance value at 10 ±0.5pf.
Embodiment 3
[0039] Others are all the same as in Example 1, the difference is that feeding starts when the capacitance value reaches 10pf, and the total flow rate of feeding is F=0.75L / h, wherein the ratio of feeding and water replenishment is adjusted according to the changing trend of the capacitance value to maintain the capacitance value at 10 ±0.5pf.
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Abstract
The invention relates to a method for the semi-continuous production of gentamicin C1a based on the control of the specific growth rate, which comprises the steps of primary seed cultivation, secondary seed cultivation and fermentation production. During the fermentation production process, the fermented liquid is fed and Water replenishment operation, while controlling the discharge of the fermentation broth, keeping the culture volume of the fermentation broth constant, and maintaining the specific rate during the fermentation process at 0.015‑0.025h ‑1 In the range. Compared with the prior art, the process of the present invention effectively maintains the metabolic activity of gentamicin C1a during the product synthesis period, prolongs the culture period to 300 hours, and greatly improves the production efficiency. The process of the present invention has determined that the specific rate in the fermentation process is maintained at 0.015-0.025h in the actual semi-continuous fermentation production process ‑1 It is most powerful for product synthesis in the range of .
Description
technical field [0001] The invention belongs to the technical field of fermentation engineering, specifically relates to the fermentation production of gentamicin C1a, in particular to a method for semi-continuous production of gentamicin C1a based on the control of specific growth rate. Background technique [0002] In the fermentation process, it is generally used to improve the yield of the product by optimizing the medium components and changing the fermentation control conditions. Among them, the specific growth rate μ is used as a key kinetic indicator of growth, not only the ratio of product synthesis rate q to the product p and closely related to the physiological metabolic state of the bacteria. Under the control of different specific growth rates, metabolites, metabolic pathways, and specific related gene expression, transcription, and protein synthesis will all change accordingly to adapt. Therefore, if the specific growth rate is used as a process control index...
Claims
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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/50C12N1/20
CPCC12N1/20C12P19/50
Inventor 李敏超陈腾飞张嗣良庄英萍储炬杭海峰
Owner QINGDAO INNOVATION INST OF EAST CHINA UNIV OF SCI & TECH
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