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A Strain of Staphylococcus Pasteur with Acetic Acid Degradation Ability and Its Application

A Staphylococcus, Pasteur's technology, applied in the direction of bacteria, microorganism-based methods, water/sludge/sewage treatment, etc., can solve the problems of disguised phase transfer, no real removal of acetic acid, purification of acidic metabolites, etc.

Active Publication Date: 2017-05-10
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These existing methods cannot purify acidic metabolites well in biological treatment, the reason is that some methods need to add other substances in the treatment process, these substances may have toxic effects on microorganisms; some methods will produce secondary Contamination or disguised transfer of contaminants without true removal of acetic acid

Method used

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  • A Strain of Staphylococcus Pasteur with Acetic Acid Degradation Ability and Its Application
  • A Strain of Staphylococcus Pasteur with Acetic Acid Degradation Ability and Its Application
  • A Strain of Staphylococcus Pasteur with Acetic Acid Degradation Ability and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Staphylococcus pasteuri Isolation, purification and identification of LWX.

[0020] 1. Staphylococcus pasteuri Isolation and purification of LWX.

[0021] After aerating the sludge of a certain urban sewage treatment plant with air for three days, take 50mL supernatant for centrifugation, place the activated sludge at the bottom in an inorganic salt medium containing only acetic acid for acclimatization and cultivation, and the concentration of acetic acid is from low to high ( 50~200mg / L), make acetic acid as the main carbon source for growth, and let it gradually adapt to the acidic environment. After domestication for about a month, measure the amount of acetate ions and total organic carbon in the water at regular intervals to determine whether the bacteria can degrade acetic acid. When the concentration of acetic acid drops obviously, take out 200mL of the mixed solution and spread it on the solid inorganic salt medium containing only acetic acid, a...

Embodiment 2

[0025] Example 2: Degradation curves of bacterial strain LWX to different concentrations of acetic acid.

[0026] The degradation of different concentrations of acetic acid by the bacterial strain LWX was investigated. The main steps were as follows: acetic acid with an initial concentration of 200, 400, 500, 800, and 1000 mg / L was added as the only carbon source in the sterilized inorganic salt medium, After the same amount of strain LWX was inoculated, the culture was sealed and shaken, and the temperature was set at 30°C. The concentration of acetic acid and the biomass of strains were detected at regular intervals.

[0027] The result is as figure 2 shown. Under the condition of 30℃, the degradation rates of strain LWX to the initial concentration of 200, 400, 500, 800 and 1000 mg / L acetic acid within 4 days were 100%, 100%, 100%, 45.94%, 0%, respectively. The strain LWX can basically degrade the low concentration of acetic acid (below 500mg / L), but the degradation eff...

Embodiment 3

[0028] Example 3: Carbon balance analysis and pH changes during degradation of acetic acid by strain LWX.

[0029] Strains analyzed Staphylococcus pasteuri Carbon balance of LWX to initial acetic acid concentration 200-1000 mg / L. The results showed that the strain LWX could convert acetic acid to CO 2 、H 2 O and cell biomass, when the concentration of acetic acid is <800mg / L, the average mineralization rate is about 70-80%, and the productivity coefficient is 0.210-0.279 mg cells / mg acetic acid. When the initial concentration of acetic acid is 200-500mg / L, the pH of the culture system tends to be neutral after the degradation is complete. The specific implementation steps are as follows:

[0030] Add acetic acid as the sole carbon source to the sterilized inorganic salt medium, set the initial concentration to 200, 400, 500, 800, and 1000 mg / L, and inoculate the same amount of strains Staphylococcus pasteuri After LWX, seal and shake culture, and set the temperature at ...

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Abstract

The present invention relates to an acetic acid degrading bacterium—Staphylococcus genera LWX and its application in degrading small molecular organic acids. The Staphylococcus genera LWX is preserved in the Chinese Type Culture Collection Center, deposit number: M 2014259. The invention can effectively process the acidic metabolites produced in the biological purification process, buffer the change of the pH value of the system, and ensure the growth activity of other microorganisms.

Description

technical field [0001] The present invention relates to a strain of Staphylococcus Pasteur ( Staphylococcus pasteuri ) LWX, and its application in microbial degradation of acetic acid. Background technique [0002] The types of pollutants that can be degraded by microorganisms can be roughly divided into polycyclic aromatic hydrocarbons, organic dyes and pigments, surfactants, pesticides, phenols, and halogenated hydrocarbons. The potential advantages of applying biodegradation principles to remediate polluted environments are now widely recognized. It is not only economical and safe, but also has a low threshold of pollutants and few residues, so its application prospect is good. [0003] The use of biological methods to treat wastewater and waste gas is becoming more and more mature. Microbial degradation of organic matter will inevitably produce acidic metabolites. The acidic metabolites are mainly fatty acids, such as formic acid, acetic acid (acetic acid), propionic a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/44C02F101/34
CPCC02F3/34C02F2101/34C12N1/205C12R2001/44
Inventor 成卓韦陈建孟於建明
Owner ZHEJIANG UNIV OF TECH
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