Method for accurately detecting content of galactooligosaccharide in milk powder

Abstract

The invention relates to a method for accurately detecting the content of galactooligosaccharide in milk powder. The method comprises the following steps: carrying out preparation by using a 3-0-beta-D-galactopyranosyl-D-glucopyranose standard substance to obtain a standard substance solution, weighing a galactooligosaccharide raw material, dissolving the galactooligosaccharide raw material in water to prepare standard curve solutions with different concentrations, carrying out ion chromatography detection on the solutions, and determining a chromatographic peak of galactooligosaccharide disaccharide in the galactooligosaccharide solution by using the chromatographic peak of the obtained 3-0-beta-D-galactopyranosyl-D-glucopyranose standard substance; and weighing a milk powder sample to prepare a sample solution, carrying out ion chromatography detection, carrying out quantification based on an external standard method by using the determined galactooligosaccharide disaccharide in thegalactooligosaccharide raw material solution as a quantitative peak and calculating a content of galactooligosaccharide in the sample solution. Therefore, a problem that the existing method cannot accurately determine the galactooligosaccharide and accurately measure the content of galactooligosaccharide in milk powder in the prior art is solved; and the method has advantages of low detection limit and accurate quantification.

Description

technical field [0001] The invention relates to the technical field of analysis and detection, in particular to a method for accurately detecting the content of galacto-oligosaccharides in milk powder. Background technique [0002] Galactooligosaccharides are oligosaccharide mixtures with different degrees of polymerization or the same degree of polymerization but containing multiple isomers of different glycosidic linkages. The molecular structure is generally 1 to 7 galactose groups connected to galactose or glucose molecules, that is, Gal -(Gal)n-Glc / Gal (n=0-6). Galacto-oligosaccharide is a functional oligosaccharide with natural properties. It can be used by probiotics such as Bifidobacteria and Lactobacillus in the human intestinal tract. It has a strong proliferation effect on intestinal probiotics and can promote gastrointestinal motility , Improve gastrointestinal function. In 2008, the Ministry of Health issued the "Announcement on the Approval of Galactooligosac...

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Problems solved by technology

[0007] The purpose of the present invention is to solve the above-mentioned problems existing in the prior art, and proposes a method for accurately detecting the content of galacto-oligosaccharides in milk powder, using 3-0-β-D-galactopyranosyl-D-pyridine Glucopyranose is used as a standard product to characterize galacto-oligosaccharide disaccharides in galacto-oligosaccharide raw materials, and then determine the content of galacto-oligosaccharides in milk powder, so as to solve the problem that existing methods cannot accurately characterize galacto-oligosaccharides and accurately measure milk powder The problem of galacto-oligosaccharide content

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