A kind of combination protein and its application

A technology for combining proteins and annexins, applied in the biological field

Active Publication Date: 2020-10-27
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is currently no commercially available vaccine for dogs (the ultimate host) to prevent E. granulosus infection in dogs

Method used

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  • A kind of combination protein and its application
  • A kind of combination protein and its application
  • A kind of combination protein and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: animal immune protection test

[0037] 1. Test groups

[0038] See Table 1.

[0039] Table 1 Grouping of test animals

[0040]

[0041]

[0042] 2. Preparation of mixed vaccine

[0043]Dilute rEgANX / rEgTIM recombinant protein and saponin Quil A with sterile PBS to make an immune mixture containing 100 μg rEgANX, 100 μg rEgTIM and 1 mg Quil A per ml; an immune mixture containing 200 μg rEgANX and 1 mg Quil A per ml; Immunization mix containing 200 μg rEgTIM and 1 mg Quil A.

[0044] 3. Immunization procedure

[0045] In the test group, 1 mL of the immune mixture was subcutaneously injected into the neck of each dog for two immunizations, and the second immunization was performed at an interval of two weeks after the first immunization.

[0046] 4. Artificial infection

[0047] Two weeks after the second immunization, artificial infection was carried out, and each Beagle dog in the test group and the control group was orally administered 70,000 p...

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Abstract

The invention relates to the field of a biological technology, and discloses combination protein and an application thereof. The combination protein comprises combination protein comprising echinococcus granulosus annexin EgANX and triose phosphate isomerase EgTIM or fusion protein granule echinococcus granulosus annexin EgANX and triose phosphate isomerase EgTIM. The granule echinococcus granulosus annexin EgANX and the triose phosphate isomerase EgTIM are used as antigranule echinococcus granulosus annexin for immunization experiment, and results show that two kinds of protein have good immunoprotection effects on dogs, can produce a stable antibody, and have the potential for developing a granule echinococcus vaccine for the dogs.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a combined protein and its application. Background technique [0002] Cystic echinococcosis, also known as cystic echinococcosis, is a worldwide zoonotic parasitic disease caused by the middle-stage larva of Echinococcus granulosus. The disease causes about 3 billion economic losses every year. The disease occurs in more than 20 provinces in my country, mainly in Qinghai, Tibet, Xinjiang and other places. It is estimated that there are about 350,000-1.3 million echinococcosis patients in China. Hydatid disease is a second-class animal disease in my country, and it is one of 16 animal diseases that are prioritized for prevention and control in my country. [0003] Echinococcus granulosus requires an intermediate host (sheep, cattle and other domestic animals) and a final host (dog) to complete its life cycle. Blocking any part of the transmission route can effectively block the trans...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/435C12N9/90C07K19/00A61K39/00A61P33/10
CPCA61K39/0003A61P33/10C07K14/43554C07K2319/00C12N9/90C12Y503/01001
Inventor 杨光友华瑞其宋宏宇
Owner SICHUAN AGRI UNIV
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