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Pharmaceutical composition with synergistic anti-melanoma effect and application thereof

An anti-melanin and composition technology, applied in the field of medicine, can solve the problems of melanoma, etc., and achieve the effect of reducing the risk of side effects, reducing the dosage of drugs, and better anti-melanoma

Active Publication Date: 2020-01-10
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Prior to 2011, the FDA-approved treatments for metastatic melanoma were dacarbazine, recombinant human interleukin (IL)-2, and high-dose or pegylated interferon (IFN)-α, but these treatments only Antitumor response in about 20% of patients
[0007] At present, there is no report on the use of cinnamaldehyde and dacarbazine to treat melanoma

Method used

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  • Pharmaceutical composition with synergistic anti-melanoma effect and application thereof
  • Pharmaceutical composition with synergistic anti-melanoma effect and application thereof
  • Pharmaceutical composition with synergistic anti-melanoma effect and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] By MTT experiment, investigate cinnamon aldehyde and dacarbazine combination (composition of the present invention, concrete proportion sees Table 1) to the proliferation inhibitory action of melanoma cell A375

[0038] Cells in the logarithmic growth phase were planted in a 96-well plate at 4000 cells / well, with a volume of 80 μL per well, and placed at 37°C, 5% CO2 Conditioned incubator overnight. After the cells adhered to the wall, the blank group was added with double distilled water, and the control group was added with equal dilution solvent DMSO, both at 20 μL / well. In the concentration setting of the combined drug group, the concentration of cinnamon aldehyde is based on the IC of different melanoma cells when acting alone 50 Value setting, after administration, it acts at 37°C, 5% CO 2 10 μL / well of each drug in a conditional incubator for 72 hours; 20 μL of MTT solution (5 mg / mL) was added to each well, placed at 37 °C for 4 h, after the supernatant was suck...

Embodiment 2

[0045] Through the MTT experiment, investigate cinnamon aldehyde and dacarbazine combination (composition of the present invention, specific proportion is shown in Table 2) to the proliferation inhibitory effect of melanoma cell A875.

[0046] Cell seeding plate, dosage concentration setting, action time, and cell viability assay methods were all based on Example 1, with 3 replicate wells for each group, and the experiment was repeated 3 times, and the average value was taken as the final result. CompuSyn software was used to analyze the CI value, 1 represented antagonistic effect.

[0047] The results showed that the combined effect of different concentrations of cinnamon aldehyde and dacarbazine, the inhibitory effect was stronger than that of the single drug, IC 50 The value is significantly reduced, and can show a synergistic inhibitory effect on melanoma cell A875 at some concentrations, * represents P figure 2 ,Table 2).

[0048] Table 2 CI value after combined action o...

Embodiment 3

[0051] Through the MTT experiment, investigate the proliferation inhibitory effect of cinnamaldehyde and dacarbazine (composition of the present invention, specific proportion see Table 3) to melanoma cell C918.

[0052] Cell seeding plate, dosage concentration setting, action time, and cell viability assay methods were all based on Example 1, with 3 replicate wells for each group, and the experiment was repeated 3 times, and the average value was taken as the final result. CompuSyn software was used to analyze the CI value, 1 represented antagonistic effect.

[0053] The results showed that the combined effect of different concentrations of cinnamon aldehyde and dacarbazine, the inhibitory effect was stronger than that of the single drug, IC 50 The value is significantly reduced, and can show a synergistic inhibitory effect on melanoma cell C918 at some concentrations, * represents P image 3 ,table 3).

[0054] Table 3 CI value after combined action of CA+DTIC in C918 cells ...

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Abstract

The invention discloses a pharmaceutical composition with a synergistic anti-melanoma effect and an application of the pharmaceutical composition, and the pharmaceutical composition comprises cinnamylaldehyde and dacarbazin. The composition disclosed by the invention has a relatively good melanoma resisting effect; the pathological experiments combined with the use of related mouse transplanted tumor models in the research of tumor pathogenesis find that cinnamaldehyde and dacarbazin are combined to achieve a strong effect of inhibiting the growth of solid tumors, and cinnamaldehyde and dacarbazin have a synergistic effect, so that the dosage of dacarbazin can be reduced, the risk of side effects caused by chemotherapeutics is reduced, and the pharmaceutical composition is applied to treatment of melanoma.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a pharmaceutical composition with synergistic anti-melanoma effect and application thereof. Background technique [0002] At present, melanoma is a malignant tumor with the highest fatality rate among skin cancers, accounting for about 80%. The treatment methods include targeted therapy and immunotherapy in addition to traditional surgery, radiotherapy and chemotherapy. Prior to 2011, the FDA-approved treatments for metastatic melanoma were dacarbazine, recombinant human interleukin (IL)-2, and high-dose or pegylated interferon (IFN)-α, but these treatments only Antitumor responses occur in approximately 20% of patients. Although targeted therapy and immunotherapy have obvious therapeutic effects on melanoma, the implementation of these methods is limited. If patients with targeted therapy quickly develop drug resistance, immunotherapy is only effective for some patients [M.Batu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4164A61K31/11A61P35/00
CPCA61K31/11A61K31/4164A61P35/00A61K2300/00
Inventor 白钢张伟怡高洁侯媛媛姜民
Owner NANKAI UNIV
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