Applications of tartary buckwheat seed coat extract in mitochondrion activity improvement, anti-aging gene expression enhancement, and protein saccharification inhibition

A technology of tartary buckwheat and extract, which is applied in the direction of anti-toxic agent, food science, drug combination, etc., and can solve the problem that the exact efficacy has yet to be further confirmed.

Pending Publication Date: 2020-01-21
TCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the method of promoting cell protection mechanism is in the development stage, although the preliminary research results show that it has anti-aging potential, its exact efficacy has yet to be further confirmed

Method used

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  • Applications of tartary buckwheat seed coat extract in mitochondrion activity improvement, anti-aging gene expression enhancement, and protein saccharification inhibition
  • Applications of tartary buckwheat seed coat extract in mitochondrion activity improvement, anti-aging gene expression enhancement, and protein saccharification inhibition
  • Applications of tartary buckwheat seed coat extract in mitochondrion activity improvement, anti-aging gene expression enhancement, and protein saccharification inhibition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Preparation of Tartary Buckwheat Seed Coat Extract

Embodiment 2

[0042] Tartary buckwheat seed coat extract enhances mitochondrial activity

[0043] Mitochondria are organelles that supply energy to cells, and their normal operation is essential for maintaining cell viability and proliferation. In order to explore the effect of buckwheat seed coat extract on mitochondrial function, this example uses flow cytometry to evaluate the changes in mitochondrial activity of human umbilical vein endothelial cells (HUVEC) after treatment with tartary buckwheat seed coat extract . First, the HUVEC cells are divided into 1×10 5 Each cell / well was seeded in each well of a 6-well plate containing 2 mL of M200 cell culture medium, and cultured at 37°C for 24 hours. Thereafter, the cells were treated with 1 mL of M200 medium containing 0.25 mg / mL or 0.125 mg / mL tartary buckwheat seed coat extract (experimental group), or only M200 medium was used to treat the cells as a control group. Cells in each group were cultured at 37°C for 24 hours and then used for ...

Embodiment 3

[0046] Expression of anti-aging genes of Tartary Buckwheat Seed Coat Extract

[0047] 3.1 Retinal pigment epithelial cells

[0048] In order to explore the effect of tartary buckwheat seed coat extract on the expression of anti-aging-related genes, qPCR was used to determine that human retinal pigment epithelial cells ARPE-19 were treated with tartary buckwheat seed coat extract, and its associated protein T complex (CCT) subunit, phosphoric acid Enzyme and tensin homologue inducible kinase 1 (PINK1), autophagy-related protein 8 (autophagy-related protein 8, ATG8), silencing information regulator 2 homologous protein 1 (SIRT1) and forkhead box protein O3 (FOXO3) Changes in genetic performance. In short, divide the cells into 1×10 5 Each cell / well was seeded in each well of a 6-well plate containing 2 mL of cell culture medium, and after culturing at 37°C for 24 hours, the cell culture medium was removed and the cells were washed with PBS solution. Thereafter, the cells were treat...

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Abstract

The invention discloses a health care application of a plant extract, in particular, applications of a tartary buckwheat seed coat extract in mitochondrion activity improvement, anti-aging gene expression enhancement, and protein saccharification inhibition. The tartary buckwheat seed coat extract is prepared by extracting tartary buckwheat seed coats by water used as a solvent. The provided extract can promote the generation of energy of cells, reduce the generation of saccharified proteins, enhance the expression of multiple anti-aging genes such as CCT, PINK1, SIRT1, FOXO3, and the like, and thus can prevent hypofunction and maintain the health of individuals.

Description

Technical field [0001] The present invention relates to the health care use of a plant extract, in particular to the use of a tartary buckwheat seed coat extract for preparing a composition for enhancing mitochondrial activity, promoting anti-aging gene expression, and inhibiting protein saccharification. Background technique [0002] With the increase of the average age of the population, having a healthy and high-quality elderly life has become one of the focuses of public attention, and scientific research on aging has also received more and more attention. Aging can be roughly defined as the decline of physiological functions over time, which is associated with many epidemic diseases, such as cardiovascular diseases, cancer, metabolic diseases, neurodegenerative diseases and so on. The aging process is complicated and affected by many factors, including diet, exercise, mental state, and genetic factors. At the molecular or cellular level, possible internal mechanisms include...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/105A23L2/38A61K36/70A61P39/06A61K131/00
CPCA23L33/105A23L2/38A61K36/70A61P39/06A23V2002/00
Inventor 林咏翔
Owner TCI CO LTD
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