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High-salinity fermentation monascus yellow pigment preparation method

A monascus yellow pigment and high-salt technology, applied in biochemical equipment and methods, fermentation, microorganism-based methods, etc., can solve problems such as limited increase in amount, and achieve the effects of low production cost, increased yield, and high yield

Active Publication Date: 2020-01-31
WUHAN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Obtaining Monascus pigment through liquid fermentation of Monascus is the focus of research, and most reports focus on optimizing medium composition and fermentation conditions to increase the content of Monascus pigment, but the amount of increase is limited

Method used

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  • High-salinity fermentation monascus yellow pigment preparation method
  • High-salinity fermentation monascus yellow pigment preparation method
  • High-salinity fermentation monascus yellow pigment preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Activation of strains: Get Monascus ruber (CGMCC 10910) strains and spread them on the plate medium for cultivation, wherein the components of the plate medium are glucose, potato, agar and water respectively, and the concentration of glucose is 20g / L , the concentration of potato is 200g / L, the concentration of agar is 20g / L, and its culture condition is: culture temperature 25-35 ℃, preferably 30 ℃; plate culture time 4-7d, preferably 6d, after the strain activation will be on the plate Bacterial colonies were formed.

[0025] Preparation of seed bacterial liquid: take 20g glucose, 3g yeast extract powder, 10g fish meal peptone, 0.5g KCl, 0.01g FeSO 4 ·7H 2 O and 4g KH 2 PO 4 Dissolve in 500mL of water, then add water to make up to 1L to obtain seed culture medium, take 80mL of seed culture medium to sterilize and cool, inoculate 13 colonies taken from the plate, and then cultivate in a constant temperature shaker at a temperature of 25 ~ 35°C, the oscillation fre...

Embodiment 2

[0027] Take 50g glucose, 5g (NH 4 ) 2 SO 4 , 5g KH 2 PO 4 , 0.5g MgSO 4 ·7H 2 O, 0.5g KCl, 0.01g FeSO 4 ·7H 2 O, 0.01g ZnSO 4 ·7H 2 O, 0.03g MnSO 4 ·H 2 O and 20g NaCl were dissolved in deionized water and made up to 1L with deionized water, followed by sterilization at 115°C for 20min. Then inoculate 80mL of the seed bacteria liquid prepared in Example 1 and carry out shaker culture, wherein the temperature of shaker culture is 30°C, the oscillation frequency is 180rpm, and the fermentation culture time is 6d; after the cultivation is completed, the fermented liquid is centrifuged (8000r / min, 5min), and the supernatant and precipitate were collected respectively, wherein the supernatant was used to obtain extracellular monascus yellow, and the precipitate was used to extract intracellular monascus yellow.

Embodiment 3

[0029] With embodiment 2, its difference is, described NaCl consumption is 35g.

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Abstract

The invention discloses a high-salinity fermentation monascus yellow pigment preparation method. The method comprises the following steps of first, preparing minimal mediums with high salinity, wherein the salt content of the minimal mediums is no less than 20g / L to 50g / L; second, taking a monascus seed solution and inoculating monascus seed in the minimal mediums prepared in the first step, and carrying out aerobic fermentation for 4 days to 7 days; and third, carrying out solid and liquid separation on the fermented broth obtained in the second step, obtaining extracellular monascus yellow pigment from serum, and obtaining intracellular monascus yellow pigment from solid with an ethanol extraction and separation method, wherein the extracellular monascus yellow pigment and intracellularmonascus yellow pigment forms a monascus yellow pigment product together. According to the high-salinity fermentation monascus yellow pigment preparation method, fermentation is carried out in the extreme environment with high salinity which can improve the capacity of monascus for generating the monascus yellow pigment. In such way, the yield of monascus yellow pigment can be obviously increased,it can be taken into consideration that seawater or concentrated seawater can be directly used for preparing the mediums for fermentation production, the whole production method is low in productioncost and friendly to the environment while being high in yield.

Description

technical field [0001] The invention belongs to the field of fermentation, and in particular relates to a preparation method of high-salt fermentation monascus yellow pigment. Background technique [0002] Monascus is a filamentous fungus that has been studied in Asian countries for thousands of years. As a secondary product of the fermentation and metabolism of Monascus, monascus pigment is a kind of natural mixed pigment with polyketide structure, which is mainly divided into three categories: red, orange and yellow. Research reports in recent years have pointed out that monascus pigments, especially monascus yellow pigments, have functional activities such as antibacterial, anticancer, antioxidative, and lipid-lowering, and can be widely used in the fields of food and medicine. Therefore, the research and development of the fermentation production of monascus yellow pigment has broad prospects and great market value. [0003] Obtaining Monascus pigment through liquid fe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/18C12N1/14C12N1/38C12R1/645
CPCC12N1/14C12N1/38C12P17/181
Inventor 陈功赵露赵喜红胡婷
Owner WUHAN INSTITUTE OF TECHNOLOGY