Cartridge for nucleic acid extraction and nucleic acid extraction method
A nucleic acid and main technology, applied in the field of nucleic acid analysis, can solve the problems of poor reproducibility, long time, complex structure and so on
Active Publication Date: 2020-01-31
SK TELECOM CO LTD
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Problems solved by technology
[0006] However, traditionally, since the processes of sample purification, cell capture, cell disruption, nucleic acid extraction, and nucleic acid amplification are performed sequentially, the entire process is time-consuming and suffers from poor reproducibility
[0007] In addition, since the equipment performing these processes requires multiple chambers for multiple unit processing, the equipment has a complicated structure and there may be a problem that the sample may be contaminated during the processing of the sample
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[0069] In order to more clearly understand the features and advantages of the present disclosure, exemplary embodiments of the present disclosure will be described in detail with reference to the accompanying drawings.
[0070] In the following description and drawings, detailed descriptions of well-known functions or configurations that may obscure the subject matter of the present disclosure will be omitted for simplicity. In addition, it is to be noted that the same components are designated by the same reference numerals throughout the drawings.
[0071] The terms and words used in the following description and appended claims are not necessarily to be interpreted in the ordinary or dictionary senses, but are defined herein as appropriate to describe the present disclosure in the best possible way terms of. These terms and words should be interpreted as meanings and concepts consistent with the technical idea of the present disclosure. The embodiments described in this...
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Abstract
The present invention relates to a cartridge for nucleic acid extraction, and a nucleic acid extraction method, the purpose thereof being to simplify a process for extracting nucleic acids from a sample, and to be applied in point-of-care testing (POCT). The cartridge, according to the present invention, comprises a chamber module, an air valve module and a liquid valve module. The chamber module:comprises a preprocessing chamber in which a sample that is inserted is pulverized and undergoes homogenization, cellular destruction and purification; and has a plurality of chambers for extractingnucleic acids from a sample. Further, the air valve module and a fluid valve module installed on the upper part and the lower part of the chamber module are connected so as to control a fluidic movement among the plurality of chambers.
Description
technical field [0001] The present disclosure relates to an apparatus and method for nucleic acid analysis, and more particularly, to a cartridge for nucleic acid extraction and a nucleic acid extraction method for extracting nucleic acid by preprocessing nucleic acid. Background technique [0002] As people's life expectancy increases and health concerns increase, the importance of genetic analysis, in vitro diagnostics, gene sequencing, etc. is gaining attention, and the demand for related products and services is gradually increasing. [0003] As a result, platforms and systems capable of performing a large number of inspections with a small number of samples in a short period of time are being introduced in the market. For example, microfluidic device platforms using microfluidic technologies such as microfluidic chips and lab-on-a-chip devices are attracting attention. Microfluidic devices include multiple microchannels and microcavities designed to allow control and h...
Claims
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IPC IPC(8): G01N35/00B01L7/00B01L3/00G01N21/64
CPCB01L3/502738B01L7/52B01L2300/0654B01L2400/043B01L3/50273B01L2400/0666B01L2400/0487B01L2200/0621B01L2300/0861B01L2300/1805B01L2300/14B01L2400/06B01L2300/0681B01L2300/0627B01L2200/10B01L3/502715B01L2200/027B01L3/502753B01L13/02C12N13/00C12N15/1013G01N1/286G01N1/34G01N2001/2866B01L2200/141B01L2300/041B01L2300/06B01L2300/123C12Q1/6844G01N21/6486
Inventor 朴珍星姜镇锡崔钲轸金宗善闵畯泓
Owner SK TELECOM CO LTD