High-survival-rate sheep fertilized ovum culture method for embryo in vitro culture

Abstract

The invention discloses a high-survival-rate sheep fertilized ovum culture method for embryo in vitro culture. The method comprises the following steps of oocyte culture, seminal fluid treatment, in vitro fertilization and fertilized ovum culture, wherein the fertilized ovum culture needs to be performed by putting fertilized ova into a culture box; and the culture box comprises a box body, a plurality of culture vessels put in the box body, an ascending and descending device used for singly ascending and descending the plurality of culture vessels, a control device for controlling the temperature, CO2 and O2 in the box body, and an isolation mechanism for realizing the isolation from the box body in the culture vessel taking or storage process. The high-survival-rate sheep fertilized ovumculture method for embryo in vitro culture has the advantages that the survival rate of the fertilized ovum is improved; and through the arrangement of the culture box, the nutritional environment inthe culture box can be maintained in the process of continuously checking the fertilized ovum.

Description

technical field [0001] The invention relates to the technical field of animal fertilized egg culture, in particular to a sheep fertilized egg culture method with high survival rate for embryo in vitro culture. Background technique [0002] With the development of modern reproductive biotechnology and the gradual improvement of embryo in vitro production technology, sheep embryo in vitro production technology (in vitro fertilization or cloning) has become an important way to speed up the breeding of breeds and the expansion of excellent populations. Although living offspring have been obtained through in vitro embryo production technology, in vitro embryos obtained by in vitro fertilization or cloning generally have developmental blockage during the culture process, that is, embryos stop developing and degenerate after reaching a certain stage, resulting in in vitro embryos. The development rate of blastocysts is much lower than that of in vivo embryos. The nutritional envir...

AI Technical Summary

Problems solved by technology

Although living offspring have been obtained through in vitro embryo production technology, in vitro embryos obtained by in vitro fertilization or cloning generally have developmental blockage during the culture process, that is, embryos stop developing and degenerate after reaching a certain stage, resulting in in vitro embryos. Blastocyst development rate is much lower than in vivo embryos

The nutritional environment of the current in vitro culture of sheep embryos is one of the key reasons for the above problems. For example, in the process of checking the fertilized eggs of sheep, it is necessary to take the fertilized eggs out of the incubator. During the taking process, the temperature in the incubator, Humidity, oxygen, carbon dioxide, etc. have great influence

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