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Urine biomarker for Japanese schistosomiasis early diagnosis, screening method, and application

A biomarker, schistosomiasis technology, applied in the field of biomedicine, can solve the problems of early diagnosis of discomfort, low specificity, low sensitivity, etc., and achieve accurate reflection and high specificity

Active Publication Date: 2020-02-04
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned diagnostic methods have low specificity or low sensitivity, and are not suitable for early diagnosis; therefore, it is necessary to find a method for early diagnosis of schistosomiasis japonicum

Method used

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  • Urine biomarker for Japanese schistosomiasis early diagnosis, screening method, and application
  • Urine biomarker for Japanese schistosomiasis early diagnosis, screening method, and application
  • Urine biomarker for Japanese schistosomiasis early diagnosis, screening method, and application

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Embodiment 1

[0036] 1. Establishment of a mouse model of Schistosoma japonicum infection

[0037]Healthy SPF-grade 8-week-old BALB / c mice were divided into seven groups: normal control group, 3-day S. The 28-day infection group and the 42-day Schistosoma japonicum infection group had 10 mice in each group. The normal control group was not treated; the mice in the infection group were infected according to the conventional method: take the positive snails and place them in dechlorinated water, release the cercariae for 1-2 hours under the light condition of 25°C-28°C, and use the inoculation loop to dip in 30 cercariae on the water surface Collected on a coverslip. After the mice were anesthetized, the abdomen was fixed on the mouse board, and the abdomen was shaved and then moistened with dechlorinated water. The coverslips stained with cercariae were attached to the abdominal skin of the mice for 20 minutes to infect.

[0038] 2. Sample collection and pretreatment

[0039] Use metaboli...

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Abstract

The invention discloses a urine biomarker for Japanese schistosomiasis early diagnosis. The urine biomarker is one or more of a xanthurenic acid, a naphthalenesulfonic acid, or enanthylcarnitine. Sensitivity and specificity of the marker are greater than 0.9, and an area under the curve (AUC) is greater than 0.9, indicating that the three indicators have better predictability and may be used for the Japanese schistosomiasis early diagnosis. The invention further discloses a metabolomics-based screening method for the urine biomarker for the Japanese schistosomiasis early diagnosis. By constructing a mouse Japanese schistosomiasis model, ultra performance liquid chromatography-tandem mass spectrometry is used to perform metabolomics analysis on mouse urine, and a characteristic differentialmetabolite, that is, the biomarker for the Japanese schistosomiasis early diagnosis, between a normal mouse and a Japanese schistosomiasis-infected mouse is found and analyzed. The screening method is non-invasive, convenient and fast, and can accurately reflect a metabolic spectrum difference between the Japanese schistosomiasis-infected mouse and the normal mouse and have high specificity.

Description

technical field [0001] The invention relates to the technical field of biomedicine, and more specifically, to a urine biomarker for early diagnosis of schistosomiasis japonicum and a screening method and application. Background technique [0002] Schistosoma japonicum is a zoonotic parasitic disease caused by Schistosoma japonicum that seriously endangers human health and hinders social and economic development. It is widely prevalent in Asia, especially in China, the Philippines and Indonesia, where more than 100 Ten thousand people are infected with the disease, and the threatened population is about 46 million people. In my country, Schistosoma japonicum is mainly prevalent in 12 provinces, municipalities and autonomous regions in the middle and lower reaches of the Yangtze River and its south. After more than 60 years of hard work, the prevention and control of schistosomiasis in my country has achieved remarkable results. Now the endemic areas are mainly distributed in...

Claims

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Application Information

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IPC IPC(8): G01N30/86G01N30/06
CPCG01N30/06G01N30/8686
Inventor 吕志跃胡玥黄萍周洪利马玉斌
Owner SUN YAT SEN UNIV
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